Objective:To determine the chlorogenic acid in stem bark and leaf of Acanthopanacis giraldii from different collection time. Methods: Chromatography parameter: kromasil C

18

（4.6 mm×250 mm

5 μm）;column temperature:30 ℃;the mobile phase: acetonitrile-（2 ‰） and phosphoric acid water（10∶90） aqueous solution;detection wavelength: 327 nm;flow rate:1 mL·min

-1

.Results: The method showed good linearity within the concentration ranges of 0.355～3.55 μg（r=（0.999 5））.The average recovery was 100.1%.The contents in the anthesis（from June to July）were lower

and the contents in the fructescence（from August to September） were higher and the content in leaf was higher than that in the bark.Conclusion: This method is simple

accurate

roducible and suitable for the determination of chlorogenic acid in stem bark and leaf of Acanthopanacis giraldii.Giving the consideration of chlorogenic acid

we suggested that the leaf of Acanthopanacis giraldii Harms can be used a new recourse of chlorogenic acid and the best collection time of Cortex Acanthopanacis giraldii were in the frutescence（August～Desember）.