中药髓复康对恒河猴急性脊髓损伤后勿动蛋白mRNA及其蛋白表达的影响

何爽; 朱嘉; 李莉; 张万强; 孔焕宇

doi:10.13422/j.cnki.syfjx.2009.02.017

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中药髓复康对恒河猴急性脊髓损伤后勿动蛋白mRNA及其蛋白表达的影响

药理 | 更新时间：2024-09-23

- 中药髓复康对恒河猴急性脊髓损伤后勿动蛋白mRNA及其蛋白表达的影响
- The Effect of Chinese Medicine Suifukang on the Expression of Nogo-A mRNA and Protein after Spinal Cord Injury in Rhesus Monkeys
- 中国实验方剂学杂志 2009年15卷第2期页码：49-51
- 作者机构：
  
  中国中医科学院望京医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2009.02.017
  中图分类号： R285.5
- 纸质出版日期：2009
- 稿件说明：
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[1]何爽,朱嘉,李莉,张万强,孔焕宇.中药髓复康对恒河猴急性脊髓损伤后勿动蛋白mRNA及其蛋白表达的影响[J].中国实验方剂学杂志,2009,15(02):49-51.

HE Shuang, ZHU Jia, LI Li, et al. The Effect of Chinese Medicine Suifukang on the Expression of Nogo-A mRNA and Protein after Spinal Cord Injury in Rhesus Monkeys[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(2): 49-51.
[1]何爽,朱嘉,李莉,张万强,孔焕宇.中药髓复康对恒河猴急性脊髓损伤后勿动蛋白mRNA及其蛋白表达的影响[J].中国实验方剂学杂志,2009,15(02):49-51. DOI： 10.13422/j.cnki.syfjx.2009.02.017.

HE Shuang, ZHU Jia, LI Li, et al. The Effect of Chinese Medicine Suifukang on the Expression of Nogo-A mRNA and Protein after Spinal Cord Injury in Rhesus Monkeys[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(2): 49-51. DOI： 10.13422/j.cnki.syfjx.2009.02.017.

摘要

目的:观察急性脊髓损伤后中药髓复康干预对勿动蛋白（Nogo-A）mRNA及其蛋白表达的影响。方法:成年雄性恒河猴14只

其中10只用于制备T12胸髓左侧半横断损伤模型

并将其随机分为髓复康组和模型组

另外4只设为正常对照组。采用原位杂交法检测各组Nogo-A mRNA的表达量;用免疫组化法检测各组Nogo-A蛋白的表达量。结果:脊髓损伤后各组Nogo-A mRNA及其蛋白的阳性细胞数及其阳性产物的OD值均明显升高

与正常组比较差异显著（P<0.05）;其中髓复康组Nogo-A mRNA及其蛋白的阳性细胞数和其阳性产物的OD值都明显低于模型组。结论:中药髓复康对脊髓损伤后恒河猴脊髓中Nogo-A的表达有一定的抑制作用

可能为神经的修复和再生提供有利的微环境。

Abstract

Objective: To investigate the effect of Chinese Medicine Suifukang（SFK） on the expression of Nogo-A mRNA and protein after spinal cord injury in rhesus monkeys.Methods: Ten out of fourteen adult rhesus monkeys received the left spinal cord hemisection on T12

and then was randomly divided into two groups: SFK groups（5） and control group（5）.The other four monkeys were served as the normal controls.The expression of Nogo-A mRNA was detected by in-situ hybridization technique and the expression of Nogo-A protein was detected by immunohistochemistry technique.Results: The positive cells of Nogo-A mRNA and its OD value in the model group were more than those in other groups（P<0.05）;The Nogo-A mRNA positive cells and its OD value in SFK group were less than those in the model group（P<0.05）.The changes of Nogo-A protein positive cells number and its OD value was similar with that of Nogo-A mRNA.Conclusions:SFK can restrain the expression of Nogo-A obviously.It is possible to improve microenvironment in the injured spinal cord and to regenerate of nervous tissue.

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