Objective: To analysis the correlation between bioactivity of inhibiting oxytocin-induced uterine contraction in vitro and volatile components of Danggui-Chuanxiong different ratio.Methods: The essential oil was extracted from Danggui-Chuanxiong by steam distillation and analyzed by GC-MS with the method of programmed temperature.Oxytocin induced female mice isolated uterine contraction model was adopted in the experiment to evaluate biological activity of Myrrh oil.Results: The experiments showed that the activities of volatile oils obtained from different ratio Danggui-Chuanxiong were different: Danggui-Chuanxiong（2∶1） > Danggui-Chuanxiong（3∶1） > Danggui-Chuanxiong（1∶1）>Danggui-Chuanxiong （3∶2）;The results of GC-MS showed that the constituents of volatile oil obtained from Danggui-Chuanxiong（2∶1） were composed with the ratios of（Z）-ligustilide:（Z）-3-butylidene phthalide: 4

5-dihydro-3-butyl-phathalide:（E）-ligustilide: Unknown compound: 8

9-dehydro-Cycloisolongifolene: Neocnidilide:（E）-3-butylidene phthalide（80.75∶4.02∶2.07∶1.66∶0.81∶0.61∶0.53∶0.52）.Conclusion: The different activities of volatile oils derived from different ratio of Danggui-Chuanxiong were resulted from the different composition of components in volatile oils.And the results suggested that the lactone-type components are possible bioactive compounds.