痔消栓的HPLC含量测定

张荣; 邓秀霞; 卫罡; 李润美; 李文周; 陈军

doi:10.13422/j.cnki.syfjx.2009.07.021

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痔消栓的HPLC含量测定

质量标准 | 更新时间：2024-09-23

- 痔消栓的HPLC含量测定
- HPLC Determination of Saponins in Zhixiao Suppositories
- 中国实验方剂学杂志 2009年15卷第7期页码：25-27
- 作者机构：
  
  1. 广州中医药大学
  2. 广东轻工职业技术学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2009.07.021
  中图分类号： R286
- 纸质出版日期：2009
- 稿件说明：
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[1]张荣,邓秀霞,卫罡,李润美,李文周,陈军.痔消栓的HPLC含量测定[J].中国实验方剂学杂志,2009,15(07):25-27.

ZHANG Rong1, Deng Xiu-xia2, WEI Gang1, et al. HPLC Determination of Saponins in Zhixiao Suppositories[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(7): 25-27.
[1]张荣,邓秀霞,卫罡,李润美,李文周,陈军.痔消栓的HPLC含量测定[J].中国实验方剂学杂志,2009,15(07):25-27. DOI： 10.13422/j.cnki.syfjx.2009.07.021.

ZHANG Rong1, Deng Xiu-xia2, WEI Gang1, et al. HPLC Determination of Saponins in Zhixiao Suppositories[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(7): 25-27. DOI： 10.13422/j.cnki.syfjx.2009.07.021.

摘要

目的:建立痔消栓的HPLC含量测定方法。方法:采用HPLC法

以Kromasil C18（4.6 mm×250 mm

5μm）色谱柱为固定相

以乙腈-0.2%磷酸水溶液为流动相

梯度洗脱

流速1.0 mL.min-1

柱温23℃

检测波长为203 nm。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1分别在0.368～2.76μg

0.676～5.07μg

0.812～6.09μg范围内线性关系良好

Abstract

Objective: To denvolop HPLC an method for the determination of the content of three saponins in Zhixiao suppositories.Methods: Analysis carriedout was performed on a Kromasil C

8 column（4.6 mm×250 mm.

5 μm）at 23 ℃.The mobile phases were methanol and 0.2% phosphoricacid solution in a gradient elution.The flow rate was set at 1.0 mL·min-1 and the ultraviolet detector was operated at 203 nm.Results: The linear ranges of notoginsenoside R

ginsenoside Rg

and ginsenoside Rb

were 0.368～2.76 μg

0.676～5.07 μg and 0.812～6.09 μg

respectively.The average recoveries of the three saponins were 98.53%

99.20% and 99.35%

respectively.The RSD（n=5）were 2.11%

1.88% and 2.38%

respectively.Conclusion: The separation is a good and the results are accurate and reproducible.It can be used for the quality control of Zhixiao suppositories.

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