三皮汤对小鼠溃疡性结肠炎模型IL-2和IL-10表达的影响

谢力; 邢之华; 蒋荣鑫; 周才秀; 周伟

doi:10.13422/j.cnki.syfjx.2009.09.022

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三皮汤对小鼠溃疡性结肠炎模型IL-2和IL-10表达的影响

药理 | 更新时间：2024-09-23

- 三皮汤对小鼠溃疡性结肠炎模型IL-2和IL-10表达的影响
- Effect of Sanpi Decotion on the Expression of IL-2 and IL-10 of Mice with Ulcerative Colitis
- 中国实验方剂学杂志 2009年15卷第9期页码：67-70
- 作者机构：
  
  1. 中南大学湘雅医院中西医结合研究所
  2. 中国中医科学院中医临床基础研究所
- 作者简介：
- 基金信息：
  
  湖南省卫生厅中医药科研基金（062005）
- DOI：10.13422/j.cnki.syfjx.2009.09.022
  中图分类号： R285.5
- 纸质出版日期：2009
- 稿件说明：
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[1]谢力,邢之华,蒋荣鑫,周才秀,周伟.三皮汤对小鼠溃疡性结肠炎模型IL-2和IL-10表达的影响[J].中国实验方剂学杂志,2009,15(09):67-70.

XIE Li~1, XING Zhi-hua~1, JIANG RONG-xin~. Effect of Sanpi Decotion on the Expression of IL-2 and IL-10 of Mice with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(9): 67-70.
[1]谢力,邢之华,蒋荣鑫,周才秀,周伟.三皮汤对小鼠溃疡性结肠炎模型IL-2和IL-10表达的影响[J].中国实验方剂学杂志,2009,15(09):67-70. DOI： 10.13422/j.cnki.syfjx.2009.09.022.

XIE Li~1, XING Zhi-hua~1, JIANG RONG-xin~. Effect of Sanpi Decotion on the Expression of IL-2 and IL-10 of Mice with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(9): 67-70. DOI： 10.13422/j.cnki.syfjx.2009.09.022.

摘要

目的:观察三皮汤对溃疡性结肠炎（UC）小鼠的疗效及探讨其治疗机制。方法:40只BALB/C小鼠随机分成正常组、模型组、柳氮磺胺吡啶（SASP）组和三皮汤组

每组10只。予以恶唑酮建立模型后

三皮汤组和SASP组分别给予三皮汤和SASP ig

每天观察小鼠体重、大便、饮食等情况

10天后处死小鼠

称脾重

取其病变结肠观察组织学改变

并采用酶联免疫吸附法（ELISA）测定各组小鼠血清白细胞介素（IL）-2及IL-10的含量。结果:三皮汤组和SASP组小鼠症状较模型组有明显改善;三皮汤组和SASP组小鼠脾组织重量较模型组有所增加;三皮汤组IL-2含量高于模型组（P<0.01）

IL-10含量低于模型组（P<0.01）。结论:三皮汤对UC有确切疗效

且能升高血清IL-2及降低IL-10的含量

调整细胞因子间的网络平衡

改善机体免疫功能

控制肠道炎症。

Abstract

Objective:To investigate the therapeutic effect of Sanpi Decotion in ulcerative colitis（UC） mice and discuss its therapeutic mechanism.Methods:Forty BALB/C mice were divided randomly and equally into normal group

model group

sulfasalazine（SASP） group

and Sanpi Decotion group（ten mice in each group）.After set up the oxazolone colitis

SASP group and Sanpi Decotion group were treated with sulfasalazine and sanpi decotion respectively

to observe the body weight

stool

food and drink situation of the mice every day.Killed all the mice on the tenth day

separated the spleen and weighed it

got the colon and evaluated the histological changes of the damaged colonic tissues

and measured the IL-2 and IL-10 concentrations in serum by using the enzyme-linked immunosorbent assay.Results: The symptom of Sanpi Decotion group and SASP group mice had great amelioration compared to model group mice

and compared to model group

the weight of spleen in Sanpi Decotion group and SASP group was increased

the concentration of IL-2 in Sanpi Decotion group was higher than that in model group（P<0.01）

and the concentration of IL-10 was lower than that in model group（P<0.01）.Conclusion:Sanpi Decotion is effective for treatment of UC and it can increase the concentration of IL-2 and decrease the IL-10 concentration

regulate the balance of the cytokines

improve the function of immunity

control the inflammation in the colon.

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