Objective:To observe the dynamic expression of L-FABP in rat nonalcoholic fatty liver disease（NAFLD） model and to discuss the role of L-FABP in the rat NAFLD.Methods:Fourty Wistar rats（200 g～220 g） were randomly divided into control group and model group.control group fed with standard diet;model group fed with high-fat diet（85% of the standard diet

13% of lard and 2% cholesterol）.The model group rat was put to death in 4

8

12 weeks seperately.Alanine aminotransferase in serum（ALT）

aspartate aminotransferase in serum（AST）

total cholesterol（TC）

triglyeride（TG）;mensurate fasting blood glucose（FBG）

Fasting insulin（FINS） were measured

and insulin sensitivity index（ISI） was calculated.HE staining was used to observe pathological changes in liver tissue;semi-quantitative reverse transcription polymerase Chain reaction（RTPCR） method was used to determine the expression of L-FABPmRNA changes.in rat liver tissue.Results:The expression of L-FABPmRNA was gradually increased in the rat liver of model group in 4

8 and 12 weeks（P<0.01）

the expression of L-FABP was gradually increased compared with the control group significantly（P<0.01）.In model group

the leral of serum ALT

AST

TC

TG was significantly higher than those in the control group（P<0.01）

while ISI decreased significantly（P<0.01）.Conclusion:Continuous 12 weeks high-fat diet can successfully replicate NAFLD rat model

and with extending the diet feeding time the model caused the expression of L-FABP increases gradually.