SUN Dong-mei, BI Xiao-li, XU Ai-li, et al. Determination of Hesperidin in Pericarpium Citri Reticulatae from Different Producing area by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(11): 1-3.
SUN Dong-mei, BI Xiao-li, XU Ai-li, et al. Determination of Hesperidin in Pericarpium Citri Reticulatae from Different Producing area by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(11): 1-3. DOI: 10.13422/j.cnki.syfjx.2009.11.005.
Objective:To determine of hesperidin in pericarpium citri reticulatae from the different producing area to estimate the quality of Pericarpium Citri Rcti culatae.Methods: The HPLC was adopted
the separation was performed on a Kromasil C
18
column(250 mm×4.6 mm
5 μm)
methanol-acetic acid-water(35∶4∶61) was used as the mobile phase
the detective wavelength was at 283 nm
the flow rate was at 1.0 mL·min
-1
and the column temperature was at room temperature.Results: The hesperidin showed a good linearity at the range of 0.4~3.6 μg
r=(0.999 8
) the average recovery was 99.28%
RSD=1.09%(n=6).The contents of hesperidin in the samples collected from different producing area were varied.Conclusion: The method can be used to estimate the quality of pericarpium citri reticulatae.