Objective:To study the effect of different concentration of macroelement

microelement and organic element on the growth and accumulation of tanshinones in hairy root of Salvia miltiorrhiza.Method: Hairy root of Salvia miltiorrhiza were cultured by liquid 6

7-V mediums with default

1/8

1/4

normal and 4

8 times of macroelement、microelement and organic element.We determined the growth and the contents of dihydrotanshinone、cryptotanshinone、tanshinoneⅠ、tanshinoneⅡ

A

by high performance liquid chromatography（HPLC） method at the same cultivation period.Result: Macroelement affect the growth and concentration of tanshinones significantly.Default macroelement can promote the accumulation of tanshinones significantly

but hold-back the growth of hairy hoot significantly.High concentration of macroelement can promote the growth of hairy root but reduced the accumulation of tanshinones significantly.Microelement and organic element can not affect the growth and accumulation of tanshinones significantly.Conclusion: Macroelement is the main factor to affect the growth and the accumulation of tanshinones in Salvia miltiorrhiza.Low concentration of macroelement can promote the accumulation of tanshinones

which can supply some foundations to study the cultivation of hairy roots and formation mechanism of the quality of different Salvia miltiorrhiza.