WANG Jin-yu, TONG Yan, WANG Zhi-min, et al. Simultaneous Determination of acetylharpagide and 8-O-acetylharpagide in Ajugae decumbens Thunb.by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(11): 26-28.
WANG Jin-yu, TONG Yan, WANG Zhi-min, et al. Simultaneous Determination of acetylharpagide and 8-O-acetylharpagide in Ajugae decumbens Thunb.by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(11): 26-28. DOI: 10.13422/j.cnki.syfjx.2009.11.014.
RP-HPLC法同时测定筋骨草药材中哈巴苷和乙酰哈巴苷含量
摘要
目的:建立高效液相色谱法同时测定筋骨草药材中哈巴苷和乙酰哈巴苷含量的方法。方法:采用高效液相色谱法
Kromasil C18色谱柱(4.6 mm×250 mm
5μm);流动相A-乙腈
B-水
梯度洗脱;流速1.0 mL.min-1
检测波长202 nm。结果:哈巴苷和乙酰哈巴苷在0.44
4
.40μg(r=0.9994)
0.1
1
0μg
(r=0.9998)范围内线性关系良好
加样回收率哈巴苷为100.93%
乙酰哈巴苷为99.81%
RSD值分别为1.93%
1.62%。结论:该方法简便、准确、重复性好
可作为该药材的质量控制方法。
Abstract
Objective:To develop a method for the determination of harpagide and 8-O-acetylharpagide in Herbal Ajugae.Methods:The determination was performed on Kromasil-C
18
(250 mm×4.6 mm
5 μm)and detected at 202 nm
the mobile phase was consisted of acetonitrile and water
gradient elution
the flow-rate was at 1.0 mL·min
-1
.Results:The calibration curves were linear in the range of 0.44 μg to 4.40 μg(r=(0.999 4)) for harpagide and 0.1 μg to 10 μg(r=(0.999 8)) for.The average recovery of harpagide was 100.93% with RSD 1.93%
and 8-O-acetylharpagide was 99.81% with RSD 1.62%.Conclusions: The method is simple
accurate
reproducible.It can be used for the quality control of Ajuga decumbens Thunb.