猪血清肝纤维化大鼠肝组织基质金属蛋白酶-9/13和基质金属蛋白酶组织抑制因子-1/2表达的动态变化及下瘀血汤对其影响

都广礼; 刘平; 王磊; 谭善忠; 龙爱华; 慕永平; 王春树

doi:10.13422/j.cnki.syfjx.2009.11.021

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猪血清肝纤维化大鼠肝组织基质金属蛋白酶-9/13和基质金属蛋白酶组织抑制因子-1/2表达的动态变化及下瘀血汤对其影响

药理 | 更新时间：2024-09-23

- 猪血清肝纤维化大鼠肝组织基质金属蛋白酶-9/13和基质金属蛋白酶组织抑制因子-1/2表达的动态变化及下瘀血汤对其影响
- Expression of Matrix Metalloproteinase 9/13（MMP-9/13）and Tissue Inhibitor of Metalloproteinase 1/2（TIMP-1/2）in Liver Fibrosis Induced by Porcine Serum and Xiayuxue Decoction on It
- 中国实验方剂学杂志 2009年15卷第11期页码：48-51
- 作者机构：
  
  上海中医药大学
- 作者简介：
- 基金信息：
  
  国家自然科学基金（30400579）
- DOI：10.13422/j.cnki.syfjx.2009.11.021
  中图分类号： R285.5
- 纸质出版日期：2009
- 稿件说明：
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[1]都广礼,刘平,王磊,谭善忠,龙爱华,慕永平,王春树.猪血清肝纤维化大鼠肝组织基质金属蛋白酶-9/13和基质金属蛋白酶组织抑制因子-1/2表达的动态变化及下瘀血汤对其影响[J].中国实验方剂学杂志,2009,15(11):48-51.

DU Guang-li, LIU Ping, WANG Lei, et al. Expression of Matrix Metalloproteinase 9/13（MMP-9/13）and Tissue Inhibitor of Metalloproteinase 1/2（TIMP-1/2）in Liver Fibrosis Induced by Porcine Serum and Xiayuxue Decoction on It[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(11): 48-51.
[1]都广礼,刘平,王磊,谭善忠,龙爱华,慕永平,王春树.猪血清肝纤维化大鼠肝组织基质金属蛋白酶-9/13和基质金属蛋白酶组织抑制因子-1/2表达的动态变化及下瘀血汤对其影响[J].中国实验方剂学杂志,2009,15(11):48-51. DOI： 10.13422/j.cnki.syfjx.2009.11.021.

DU Guang-li, LIU Ping, WANG Lei, et al. Expression of Matrix Metalloproteinase 9/13（MMP-9/13）and Tissue Inhibitor of Metalloproteinase 1/2（TIMP-1/2）in Liver Fibrosis Induced by Porcine Serum and Xiayuxue Decoction on It[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(11): 48-51. DOI： 10.13422/j.cnki.syfjx.2009.11.021.

摘要

目的:研究免疫性肝纤维化大鼠肝组织基质金属蛋白酶9/13和基质金属蛋白酶组织抑制因子1/2表达的动态变化及下瘀血汤对其影响

探讨下瘀血汤抗肝纤维化的机制。方法:猪血清腹腔复制大鼠肝纤维化模型

造模8周后ig下瘀血汤的流浸膏

用药4周后和在造模过程中的1、24、、8周动态处死大鼠

获取标本

检测指标。结果:MMP-2/9的高活性和TIMP-1的高表达贯穿于本模型的始终。MMP-9/13蛋白表达在肝纤维化发生发展过程中有先抑制后升高

再次抑制而后升高的反复过程

并受TIMP-1/2表达影响;下瘀血汤可以提高MMP-9活性

促进MMP-9/13而抑制TIMP-1

2表达。结论:该模型有MMP-9/13和TIMP-1/2表达的失衡;下瘀血汤可通过调控MMp-9/13和TIMP-1/2的表达

降解过度沉积的细胞外基质而发挥抗肝纤维化的作用。

Abstract

Objective:Changes of protein expression of MMP-9/13 and TIMP1/2 in fibrotic liver tissue induced by porcine serum and effect of Xiayuxue Decoction on it are investigated in this study to explore mechanism of Xiayuxue Decotion in reversing it.Methods:Liver fibrosis in rats was induced by intraperitoneal injection of porcine serum.After 8wk of modeling

rats were given Xiayuxue Decoction for 4 weeks.Rats were sacrificed and sample was obtained for determination at 1 wk

2 wk

4 wk

8 wk and the end of 12 wk of modeling for determination.Results:High activity of MMP-2/9 and high expression TIMP-1 characterizes this liver fibrosis model from beginning to the end.Decreased protein expression of MMP-9/13 in early stage and increased protein expression of MMP-9/13 in early stage and increased protein expression of MMP-9/13 affect by protein expression TIMP-1/2.Xiayuxue Decoction can increase activity of MMP-9

promote expression of MMP-9/13

inhibit expression of TIMP-1/2.Conclusions:Imbalanced MMP-9/13 and TIMP-1/2 expression is seen in this model.The anti-liver fibrosis mechanism of Xiayuxue Decoction is regulating MMP-9/13 and TIMP-1/2 expression to degrade excessive deposition of extracellular matrix.

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