LI Dong~1, CHEN Zhi-wu~2, CEN De-yi~2, et al. The Study of Anti-bradycardic Effect and Mechanism of Ganfu Granule in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(1): 68-71.
LI Dong~1, CHEN Zhi-wu~2, CEN De-yi~2, et al. The Study of Anti-bradycardic Effect and Mechanism of Ganfu Granule in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(1): 68-71. DOI: 10.13422/j.cnki.syfjx.2010.01.035.
Objective:To study the anti-bradycardic mechanism of Ganfu granule in rats.Methods: The bradycardic model in rats was induced by propranolol(Pro). the activity of glutathione peroxidase(GSH-PX)
superoxide dismutase(SOD) and the content of Malonydialdehyde(MDA) in heart tissue were detected;the content of cyclic adenosine monophosphate(cAMP)
cyclic guanosine monophosphate(cGMP) in heart tissue as well as the content of calcitonin gene-related peptide(CGRP)
endothelin(ET) in plasma were detected by radioimmunoassay.Results: Ganfu granule(30
90
270 mg·kg
-1
) significantly inhibited the Pro-induced decrease of heart rate in rats
reduced the content of MDA
cGMP in heart tissue
markedly increased the content of CGRP in plasma and the ratio of cAMP/cGMP in heart tissue. Gan granule(90
270 mg·kg
-1
) markedly increased the activity of SOD and GSH-PX in heart tissue;Compare with model group
the changes of the content of ET in plasma and cAMP in heart tissue were not obviouse.Conclusion:The anti-bradycardic mechanism of Ganfu granule may be related to anti-free radicals