清热化瘀滋阴方对狼疮患者外周血淋巴细胞凋亡及相关基因表达的影响

梁卫; 蔡鹰; 张丽玲; 唐永明; 龚林; 金琳; 蒋克春

doi:10.13422/j.cnki.syfjx.2010.02.004

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清热化瘀滋阴方对狼疮患者外周血淋巴细胞凋亡及相关基因表达的影响

临床 | 更新时间：2024-09-23

- 清热化瘀滋阴方对狼疮患者外周血淋巴细胞凋亡及相关基因表达的影响
- The Effect of Qingrehuayuziyin Prescription on Lymphocyte Apoptosis and Expression of Fas and Bcl-2 in Peripheral Blood in the Patients with Systemic Lupus Erythematosus
- 中国实验方剂学杂志 2010年16卷第2期页码：116-119
- 作者机构：
  
  中国人民解放军第454医院中医科
- 作者简介：
- 基金信息：
  
  南京军区“十一五”攻关课题资助项目（06MA73）
- DOI：10.13422/j.cnki.syfjx.2010.02.004
  中图分类号： R259
- 纸质出版日期：2010
- 稿件说明：
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[1]梁卫,蔡鹰,张丽玲,唐永明,龚林,金琳,蒋克春.清热化瘀滋阴方对狼疮患者外周血淋巴细胞凋亡及相关基因表达的影响[J].中国实验方剂学杂志,2010,16(02):116-119.

LIANG Wei, CAI Ying, ZHANG Li-ling, et al. The Effect of Qingrehuayuziyin Prescription on Lymphocyte Apoptosis and Expression of Fas and Bcl-2 in Peripheral Blood in the Patients with Systemic Lupus Erythematosus[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 116-119.
[1]梁卫,蔡鹰,张丽玲,唐永明,龚林,金琳,蒋克春.清热化瘀滋阴方对狼疮患者外周血淋巴细胞凋亡及相关基因表达的影响[J].中国实验方剂学杂志,2010,16(02):116-119. DOI： 10.13422/j.cnki.syfjx.2010.02.004.

LIANG Wei, CAI Ying, ZHANG Li-ling, et al. The Effect of Qingrehuayuziyin Prescription on Lymphocyte Apoptosis and Expression of Fas and Bcl-2 in Peripheral Blood in the Patients with Systemic Lupus Erythematosus[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 116-119. DOI： 10.13422/j.cnki.syfjx.2010.02.004.

摘要

目的:探讨中药清热化瘀滋阴方对系统性红斑狼疮（SLE）患者外周血淋巴细胞凋亡及相关基因（Fas

Bcl-2）表达的影响。方法:临床筛选SLE患者80例

完全随机分为治疗组（清热化瘀滋阴方配合激素组）和对照组（单用激素组）各40例

服药3个月

淋巴细胞分离液分离治疗前后SLE患者外周血淋巴细胞

采用流式细胞仪检测淋巴细胞凋亡率;采用Rt-PCR法检测淋巴细胞Fas

Bcl-2基因表达情况。结果:清热化瘀滋阴方治疗后SLE患者外周血淋巴细胞凋亡率较治疗前明显下降（P<0.01）

并明显低于对照组（P<0.01）;两组治疗后Fas

Bcl-2基因的表达均明显下降

治疗组Fas基因的表达明显低于对照组（P<0.01）。结论:清热化瘀滋阴方可能通过降低SLE患者外周血淋巴细胞凋亡率

下调淋巴细胞Fas

Bcl-2基因的表达水平

改善免疫紊乱而发挥作用。

Abstract

Objective:To investigate the effect of Qingrehuayuziyin prescription on lymphocyte apoptosis and expression of Fas and Bcl-2 in peripheral blood in the patients with systemic lupus erythematosus（SLE）.Methods: Eighty patients with SLE were selected and divided randomly into two groups

40 in the therapy group and 40 in control group respectively.Patients were got the treatment of Qingrehuayuziyin prescription combined hormone as therapy group and got hormone treating only as control group.After treating three months

the patients lymphocyte were collected and the rate of apoptosis were detected by flow cytometer.Meanwhile

the expression of Fas and Bcl-2 were analyzed by RT-PCR.Results: After 3 months treating

the rate of lymphocyte apoptosis was decreased significantly in therapy group compared with that in control group and with that in pre-treating（P<0.01）.The expression of Fas and Bcl-2

compared with that in control group

were also decreased significantly（P<0.01）.Conclusion: Qingrehuayuziyin prescription is useful for treating SLE by suppressing lymphocyte apoptosis

decreasing the level of Fas and Bcl-2 in peripheral blood and further modifying the immune status.

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