WANG Ping~. Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 28-31.
WANG Ping~. Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 28-31. DOI: 10.13422/j.cnki.syfjx.2010.02.012.
基于HPLC-DAD-MS的当归补血汤化学成分分析
摘要
目的:分析中药复方当归补血汤的化学成分。方法:采用HPLC-DAD-MS方法分析。色谱柱为Thermo Hypersil-HypurityC18(150 mm×2.1 mm
Objective:To study chemical components of Dangguibuxue decoction.Methods:The compounds were separated on a Hypersil-Hypurity C
18
(150 mm×2.1 mm
5 μm) analytical column.The column temperature was set at 40 ℃.Mobile phase consisted of methanol and water using a gradient elution.The flow rate was set at 0.2 mL·min
-1
.UV spectra were obtained by scanning from 200 nm to 370 nm.The APCI-MS spectra were obtained in the positive ion mode scanning from m/z 100 to 650.Results:The peaks were identified by comparing their UV spectra
their molecular weights and their retention behaviors with data reported in the literature.The ten components seem to be tentatively identified.Conclusions: Because of providing UV and molecular mass as well as retention time
LC-DAD-MS is a powerful tool for the chemical analysis of Traditional Chinese Medicines.