LI Shu-wen~. The Experimental Study on the mRNA Expression of CYP11B2 in Rat with Kidney Deficiency and Diuresis Induced by Adenine[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 64-67.
LI Shu-wen~. The Experimental Study on the mRNA Expression of CYP11B2 in Rat with Kidney Deficiency and Diuresis Induced by Adenine[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 64-67. DOI: 10.13422/j.cnki.syfjx.2010.02.034.
Objective:To explore the mechanism for kidney deficiency and diuresis by studying the action of adenine to ALD synthase.Methods:The contents of Cort
ALD in blood were determined and the mRNA expression of CYP11B2 was evaluated with ELISA and RT-PCR in model group and control group.Results:Compared with control group
adenine could remarkedly decrease the contents of Cort
ALD in rat blood;and downregulate the mRNA expression of CYP11B2.Conclusion:Adenine can decrease the contents of Cort
ALD in blood and downregulate the mRNA expression of CYP11B2 in rats.It can decrease the synthesis of ALD by adjusting the ALD synthase.The results may expound the mechanism of kidney deficiency and diuresis induced by adenine from the angle of ALD synthase approach.