ZHONG Ying-jie, LI Liang, XU Fu-liang. Studies on extraction process of cichoric acid in Echinacea purpurea[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 1-4.
ZHONG Ying-jie, LI Liang, XU Fu-liang. Studies on extraction process of cichoric acid in Echinacea purpurea[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 1-4. DOI: 10.13422/j.cnki.syfjx.2010.04.011.
紫锥菊中菊苣酸的提取工艺和含量测定
摘要
目的:优选紫锥菊药材地上部分的提取工艺
建立菊苣酸的含量测定方法。方法:择L9(34)正交表安排试验
以有效成分菊苣酸的含量为指标优选工艺参数。结果:紫锥菊的最佳提取工艺为12倍量60%乙醇提取3次
每次1.5 h。菊苣酸线性范围在0.011~1.100 mg
r=0.999 8
平均回收率99.51%(RSD=1.01%
n=6)。结论:工艺稳定、可行
适合工业化生产;含量测定方法简便准确
重复性好
可用于控制紫锥菊的质量。
Abstract
Objective:To establish the optimal extraction technology and to determine the quantity of ci-choric acid from Echinacea purpurea.Method: Nine experiments were carried out through L9(34) orthogonal de-sign.The contents of cichoric acid were determined by HPLC.Result: The best extraction condition obtained was 12 times amount 60% alcohol and extracted for 3 times
1.5 hours each time.Cichoric acid was linear within the range of 0.011 ~ 1.100 mg
r = 0.999 8.The average recovery is 99.51%(RSD = 1.01%
n = 6).Conclusion: This optimal technology is stable and workable
can be used in industrial production.The determination method is suitable for quality control.