Objective:To establish the quality standard of Huanglianjiedu Hydrogel.Method: The high performance liquid chromatography was developed for the determination the contention of jasminoidin

berberine and baicalin;Luna C18 column（4.6 mm × 250 mm

5 μm） was used in the determination and the mobile phase of jasminoidin

berberine and baicalin were a mixture of acetonitrile-water（13 ∶ 87）

acetonitrile-0.1 mol.L-1 K2HPO4（30∶ 70）

and methanol-water-phosphoric acid（47∶ 53∶ 0.2） respectively.Result: The average content of jasminoidin was no less than 0.4%

and the average recovery and RSD were 97.21% and 1.5% respectively;The average content of berberine was no less than 1.2%

and average recovery and RSD were 99.11% and 2.45% respectively;The average content of jasminoidin was no less than 1.4%

and the average recovery and RSD were 98.74% and 1.6% respectively;Conclusion: This method can be adopted as a standard for the quality control of Huanglianjiedu Hydrogel.