BI Xiao-li, XU Ai-li, LUO Wen-hui. Determination of Chonglou saponin Ⅰ and Chonglou sa ponin Ⅱ in Qingyan capsule by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 64-66.
BI Xiao-li, XU Ai-li, LUO Wen-hui. Determination of Chonglou saponin Ⅰ and Chonglou sa ponin Ⅱ in Qingyan capsule by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 64-66. DOI: 10.13422/j.cnki.syfjx.2010.04.032.
Objective: To develop an HPLC-ELSD method for the content determination of Chonglou SaponinⅠand Chonglou Saponin Ⅱin Qingyan capsule.Method:The analysis was carried out on a Hypersil C18 analytical column(4.6 mm × 250 mm
5 μm) with the mobile phase consisting of acetonitrile-water(48∶ 52).The detector drift tube temperature was set at 45 ℃ and the nitrogen flow rate was 1.5L.min-1.Result: The recoveries for Chonglou SaponinⅠand Chonglou Saponin Ⅱ were 97.27% and 97.09% with RSD 1.22% and 1.40%(n = 6)
respectively.Conclusion: The developed method is simple
rapid
replicable
and can be used for the quality control of Qingyan capsule.