WANG Ling1, GONG Mu-xin1, WANG Zhi-min3, et al. Salviae Miltiorrhizae Radix et Rhizoma by Infrared Spectroscopy[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 34-37.
WANG Ling1, GONG Mu-xin1, WANG Zhi-min3, et al. Salviae Miltiorrhizae Radix et Rhizoma by Infrared Spectroscopy[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 34-37. DOI: 10.13422/j.cnki.syfjx.2010.05.048.
不同产地丹参药材红外光谱分析
摘要
目的:建立红外光谱技术
快速鉴别丹参药材产地及确定丹酚酸B含量高低。方法:采集来自4个产地的40批丹参药材的红外光谱信息
寻找可以反映产地特征的二阶导数红外光谱特征峰
以及可进行丹酚酸B快速定量的原谱特征峰。结果:不同产地丹参药材的二阶导数红外光谱特征吸收峰存在差异
可以反映丹参药材的不同产地;原谱1262cm-1峰的吸收峰高基本不受其他成分的影响
可以反映丹酚酸B含量且与液相色谱含量结果呈正相关
实现丹酚酸B的快速半定量。结论:建立的红外光谱技术可以为中成药生产过程中
投料丹参药材的快速评价提供一种新的备选方法。
Abstract
Objective: To establish a fast method to evaluate the geographic origins of salviae miltiorrhizae radix et rhizoma and the contents of salvianolic acid B based on infrared spectroscopy (IR). Method: IR spectra of forty samples collected from 4 different origins were analyzed to search for the characteristic peaks on second derivative (2D) spectra to reflect the characteristics of origin
also to search for a characteristic peak on normalized spectra for fast quantitation of salvianolic acid B. Result: Some characteristic peaks on 2D spectra were found which reflect 4 different origins. The intensity of 1 262 cm -1 on normalized spectra was found to correlate well with the content of salvianolic acid B determined by high performance liquid chromatography
which realize the fast quantitative analysis of salvianolic acid B. Conclusion: The proposed IR method provides a new alternative of fast quality evaluation of salviae miltiorrhizae radix et rhizoma for manufacture of traditional Chinese medicine.