WANG Hong-zhi, QIAO Xiao-li, LI Wan-qing, et al. Orthogonal Design for Optimizing the Semi-bionic Extraction of Kangxuanyun Granules[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 19-24.
WANG Hong-zhi, QIAO Xiao-li, LI Wan-qing, et al. Orthogonal Design for Optimizing the Semi-bionic Extraction of Kangxuanyun Granules[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 19-24. DOI: 10.13422/j.cnki.syfjx.2010.05.051.
Objective:To investigate the method for using semi-bionic (SBE) on Kangxuanyun granules as an example. Method: Orthogonal design was used to optimize the extraction conditions of SBE with the evaluation markers such as alisol B-acetate
tetramethylpyrazine
ferulic acid
total alkaloids
the total contents of emodin and chrysophanol
dried extract. Result: The optimized SBE conditions were as follows: The pH of the three extractions were in proper order 2. 00
7. 50
8. 50; the solvent volume was 10
8
6 times; and the extraction time was 2. 0
1. 5
1. 5 h
respectively. Conclusion: The optimum extraction conditions are stable and practicable