Objective: To investigate the action of Wuling Capsules prescription ingredients and herbal withdrawal prescription and their effects on function of the original prescription. Method: The mouse model of hepatic fibrosis was established by multiple subcutaneous injection of carbon tetrachloride drinking 10% alcohol water solution

feeding hyperliposis-low protein diet and treated with experiment drugs for 10 days. The activities of ALT and CHE in serum of mice were detected by enzyme method. HSC-T6 cell was activated by transforming growth factor-beta1 in vitro

effects of the prescription ingredients and withdrawal drug compatibility on the expression of Ras /ERK and TGF-β /Smad signaling pathway pretein in HSC-T6 cell were observed. The expression of α-SMA

Ras

CollagenⅠ

ERK

p-ERK

TβRⅠ

TβRⅡ

Smad2 /3 were determined by western bloting. Result:The activity of ALT in surum of hepatic fibrisis mice was decreased by WuLing Capsules

prescription ingredients and withdrawal drug compatibility groups

while Bupleurum chinensis DC and Ganoderma group had no effects. WuLing Capsules

prescription ingrediets and withdrawal drug group had different biological effect on the protein expression of α-SMA

Collagen

ERK

p-ERK

TβRⅠ

TβRⅡ

Smad2 /3 in HSC-T6 cell induced by TGF-β1. Conclusion:Prescription ingredients compatibility produces additive effects on WuLing Capsules therapy for hepaticfibrosis mice

they inhibit the protein expression of ERK and TGF-β1 /Smad signaling pathway in HSC-T6 by TGF-β1 activation

subsequently influence the transcription and synthesis of collagenⅠin HSC-T6.