Objective:To study the wine-processed techniques of venenum bufonis and screen out the optimum processing technique.Method:The orthogonal test was applied to study the wine-processed techniques.HPLC fingerprint was used to determine the total retention rate of cinobufagin and resibufogenin.Result:Relative importance of factors affecting wine-processed techniques were as follow:processing time > processing temperature > ratio of botanical and auxiliary materials.The processing time had a remarkable effect.Chemical compositions decreased after venenum bufonis was processed by wine.The optimum processing technique was that venenum bufonis was processed at 60 ℃ by 55% ethanol and ratio of 1∶ 2 for 12 h.Conclusion:The method is steady with a simple operation and can provide a basis to set up a standardized wine-processed technique of venenum bufonis.