Objective:To bulid the method for determination of baicalin

jasminoidin and paeonol in Qindan Particles by HPLC.Method:An Aigilent ZORBAX SB-C18 column（4.6 mm × 150 mm

5 μm） was used.The mobile phase of baicalin was CH3OH-H2O-P（47∶ 53∶ 0.2）

the detection wave lenghth was set at 280 nm.And the mobile phase of jasminoidin was acetonitrile-H2O（15 ∶ 85）

the detection wave lenghth was set at 238 nm.For paeonol

the mobile phase was CH3OH-H2O（45∶ 55）

the detection wave lenghth was set at 274 nm.All flow rates were 1.0 mL.min-1.Result:Under each condition

the calibration curves of baicalin

jasminoidin and paeonol were linear at the ranges of 0.006 ～ 0.096 mg.L-1

0.005 ～ 0.080 mg.L-1 and 0.005 ～ 0.050 mg.mL-1

respectively.And the each average recoveries of the method were 98.8% for baicalin（RSD 0.75%

n = 9）;99.7% for jasminoidin（RSD 0.94%

n = 9）;98.4% for paeonol（RSD 1.41%

n = 9）.Conclusion:The developed method is simple

accurate and repeatable for determination of baicalin

jasminoidin and paeonol in Qin-dan Particles.