ZHU Wei-feng, LUO Ling-ying, ZUO Zheng-yun, et al. Determination of Oleanolic Acid in Erzhi Pills by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 101-102.
ZHU Wei-feng, LUO Ling-ying, ZUO Zheng-yun, et al. Determination of Oleanolic Acid in Erzhi Pills by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 101-102. DOI: 10.13422/j.cnki.syfjx.2010.06.064.
RP-HPLC法测定二至丸中齐墩果酸的含量
摘要
目的:建立二至丸中齐墩果酸的含量测定的方法。方法:采用Venusil X B P-C18柱(4.6 mm×250 mm
Objective:To develop a RP-HPLC method to determine the contents of oleanolic acid in ErzhiPills.Method:The samples was separated on a Venusil X B P-C18 column(4.6 mm × 250 mm
5 μm)
using methanol-0.4 % ammonium acetate solution(83 ∶ 17) as the mobile phase with flow rate of 1 mL.min-1 and detection at 210 nm.The column temperature was maintained at 25 ℃.Result:the linear range of oleanolic acid was at 0.1004 ~ 1.807 μg(r = 0.999 8).The average recovery of oleanolic acid was 99.7%
RSD 1.75%(n=6).Conclusion:The method is simple
accurate
and can be used for quality evaluation of Erzhi Pills.