ZHOU Yong-ming1, PIAO Bing-kui1, ZHENG Hong-gang1, et al. Investigation on Migration Ability and Mechanism of Dendritic Cells by Feiliuping Extract and its Modified Formula[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 152-155.
ZHOU Yong-ming1, PIAO Bing-kui1, ZHENG Hong-gang1, et al. Investigation on Migration Ability and Mechanism of Dendritic Cells by Feiliuping Extract and its Modified Formula[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 152-155. DOI: 10.13422/j.cnki.syfjx.2010.06.080.
Objective:To observe the migration ability and mechanism of dendritic cells(DC) by Feiliuping extract and its modified formula.Method:The migration ability of DCs were determinated by transwell bordens
the expression of CCR7
CXCR4 on surface of DC were detected by RT-PCR.Result:Different TCM has different influence on migration ability of DCs.And ability was different with different chemotactic factor.The expression of CCR7
CXCR4 on surface of DC were different with different TCM
CCR7 in Yiqi group was the highest
while Huoxue group
FLP group lower
Jiedu group is the lowest.Comparing with Yiqi group
Huoxue group & Jiedu group(P < 0.05).CXCR4 in Yiqi Group & FLP group was highest
Huoxue group is lower
Jiedu group is the lowest.Comparing with blank serum group and FLP group
Yiqi group
Jiedu group(P < 0.01).Conclusion:Yiqi herbs strengthens migration ability of DCs
while Jiedu herbs inhibits.The difference was related to the expression chemotactic factor receptor on DCs surface.