Objective:To investigate the influences of Tangbikang on the serum nerve growth factor（NGF） concentration and NGF mRNA expression in sciatic nerve in rats with diabetic penipheral neuropathy（DPN）.Method:70 SD rats were randomly divided into two groups:normal control 10 and diabetic penipheral neuropathy 60 by weight.Diabetes was induced by intraperitoneal injection of STZ

55 mg.kg-1.The diabetic models were affirmed upon blood glucose ≥16.7 mmol.L-1.And the diabetic rats were randomly divided into five groups:model group

methycobal treatment group

Tangbikang different dose treatment groups.After drug administration for 8 days

all the rats were sacrificed at week 8;The blood serum was obtained and NGF were detected;The sciatic nerve NGF gene was detected by the Real-time fluorescence quantitative polymerase chain reaction（PCR）.All statistical calculations were made using the SPSS16.0 statistical package.Result:After the treatment by the middle and high dose of Tangbikang

the NGF level（P < 0.01

P < 0.01） in the blood serum increased greatly.The expression of NGF gene incresed（P < 0.01）.Conclusion:Tangbikang can increase the NGF protein and gene expression to play an important role of peripheral nerve protection.