Objective:Proteomics approaches were performed to identify proteins that may be involved in dimethylnitrusamine（DMN）-induced cirrhosis /fibrosis and the effects of Huangqi Tang（HQT） intervention were observed in rats.Method:Differential protein spots that exhibited changes in HQT group in DMN-induced cirrhosis were selected by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption /ionization time-of-flight and peptide mass fingerprinting analysis.After that

these protein spots were recognized by bioinformatics information retrieval.Lastly

the results of this proteomic analysis were confirmed by immunoblotting and detection of enzyme activity.Result:Eighteen protein spots were found to be differentially expressed

including 7 spots involved in disorder of lipid metabolism and oxidative damage

3 spots involved in protein metabolism

2spots involved in biotransformation and 3 spots involved in material synthesis and energy metabolism in liver.Among them

3 spots for confirmation（Prdx6

Hsp70

CAT） were consistent with the results of two-dimensional gel electrophoresis.Conclusion:There are significant oxidative stresses occurred during hepatofibrogenesis of DMN-induced cirrhosis/fibrosis in rats and HQT can significantly improve the antioxidant capacity of liver and protect peroxide damage of cells.