HPLC测定舒筋活络酒中升麻苷和5-O-甲基维斯阿米醇苷的含量

黄捷; 朱雪妍; 罗轶

doi:10.13422/j.cnki.syfjx.2010.12.022

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HPLC测定舒筋活络酒中升麻苷和5-O-甲基维斯阿米醇苷的含量

化学与分析 | 更新时间：2024-09-23

- HPLC测定舒筋活络酒中升麻苷和5-O-甲基维斯阿米醇苷的含量
- Content Determination of Prim-O-glucosylcimifugin and 5-O-methylvisamminoside in Shujin Huoluo Wine by HPLC
- 中国实验方剂学杂志 2010年16卷第12期页码：64-66
- 作者机构：
  
  广西壮族自治区食品药品检验所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.12.022
  中图分类号： R286.0
- 纸质出版日期：2010
- 稿件说明：
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[1]黄捷,朱雪妍,罗轶.HPLC测定舒筋活络酒中升麻苷和5-O-甲基维斯阿米醇苷的含量[J].中国实验方剂学杂志,2010,16(12):64-66.

HUANG Jie, ZHU Xue-yan, LUO Yi. Content Determination of Prim-O-glucosylcimifugin and 5-O-methylvisamminoside in Shujin Huoluo Wine by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 64-66.
[1]黄捷,朱雪妍,罗轶.HPLC测定舒筋活络酒中升麻苷和5-O-甲基维斯阿米醇苷的含量[J].中国实验方剂学杂志,2010,16(12):64-66. DOI： 10.13422/j.cnki.syfjx.2010.12.022.

HUANG Jie, ZHU Xue-yan, LUO Yi. Content Determination of Prim-O-glucosylcimifugin and 5-O-methylvisamminoside in Shujin Huoluo Wine by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 64-66. DOI： 10.13422/j.cnki.syfjx.2010.12.022.

摘要

目的:建立舒筋活络酒中升麻苷和5-O-甲基维斯阿米醇苷的测定方法

以控制制剂的质量。方法:采用HPLC测定舒筋活络酒中升麻苷和5-O-甲基维斯阿米醇苷的含量。Kromasil C18柱（4.6 mm×250 mm

5μm）;流动相乙腈-0.05%磷酸溶液为流动相梯度洗脱;检测波长254 nm;流速1.0 mL·min-1;柱温35℃

进样量为10μL。结果:升麻苷在0.030 1～1.504 5μg

5-O-甲基维斯阿米醇苷进样量在0.040 8～2.040 0μg

与升麻苷和5-O-甲基维斯阿米醇苷总量峰面积呈良好的线性关系

平均回收率为升麻苷99.46%

RSD 2.64%

5-O-甲基维斯阿米醇苷100.45%

RSD 2.54%（n=9）。结论:本方法简便、快速、准确

可作为该制剂的定量分析方法。

Abstract

Objective: The content determination was established for analysis of prim-O-glucosylcimifugin and 5-O-methylvisamminoside to control the quanlity of Shujin Huoluo Wine.Method: Kromasil C18 column（5 μm

4.6 mm × 250 mm） was used with a mixture of acetonitrile and 0.05% solution of phosphoric acid as the mobile phase

gradient elution was performed.The flow rate was 1.0 mL.min-1

detection wavelength was set at 254 nm

column temperature was set at 35 ℃

the quantity of injection was 10 μL.Result: The linear range of prim-O-glucosylcimifugin and 5-O-methylvisamminoside was 0.030 1-1.504 5 μg and 0.040 8-2.040 0 μg respectively.The average recovery of prim-O-glucosylcimifugin and 5-O-methylvisamminoside was 99.46% and 100.45% with RSD 2.64% and 2.54%

respectively.Conclusion: The method is simple

rapid and with good reproducibility on the content determination in Shujin Huoluo Wine.

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