香青兰中总黄酮和单体的含量测定

宋睿; 金传山; 周亚伟

doi:10.13422/j.cnki.syfjx.2010.12.023

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香青兰中总黄酮和单体的含量测定

化学与分析 | 更新时间：2024-09-23

- 香青兰中总黄酮和单体的含量测定
- To Determine Content of Total Flavonoids and Robinin-7-o-β-D-xylosylglucoside from Dracoephalum mololarie
- 中国实验方剂学杂志 2010年16卷第12期页码：71-74
- 作者机构：
  
  1. 安徽中医学院药学院
  2. 北大世佳研究中心
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.12.023
  中图分类号： R284.1
- 纸质出版日期：2010
- 稿件说明：
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[1]宋睿,金传山,周亚伟.香青兰中总黄酮和单体的含量测定[J].中国实验方剂学杂志,2010,16(12):71-74.

SONG Rui1, JIN Chuan-shan1, ZHOU Ya-wei2. To Determine Content of Total Flavonoids and Robinin-7-o-β-D-xylosylglucoside from Dracoephalum mololarie[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 71-74.
[1]宋睿,金传山,周亚伟.香青兰中总黄酮和单体的含量测定[J].中国实验方剂学杂志,2010,16(12):71-74. DOI： 10.13422/j.cnki.syfjx.2010.12.023.

SONG Rui1, JIN Chuan-shan1, ZHOU Ya-wei2. To Determine Content of Total Flavonoids and Robinin-7-o-β-D-xylosylglucoside from Dracoephalum mololarie[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 71-74. DOI： 10.13422/j.cnki.syfjx.2010.12.023.

摘要

目的:考察香青兰中单体（刺槐素-7-o-β-D-葡萄糖苷）、总黄酮的含量。方法:采用紫外分光光度法（UV）测定总黄酮含量;采用高效液相色谱法（HPLC）测定单体含量

RP-C18柱（4.6 mm×250 mm

5μm）

流动相为乙腈-0.1%磷酸水（26∶74）

流速为1.1 mL·min-1

柱温室温

检测波长为326 nm。结果:单体（刺槐素-7-o-β-D-葡萄糖苷）含量为0.33%

回收率为98.62%;总黄酮含量为1.90%

回收率为97.59%。结论:所建方法准确、简便

为科学地评价香青兰药材的质量提供依据。

Abstract

Objective: To determine the content of total flavonoids and robinin-7-o-β-D-xylosylglucoside fron Dracoephalum mololarie.Method: The content of total flavonoids was determined by ultraviolet spectrophotometry（UV）.The content of robinin-7-o-β-D-xylosylglucoside was determined by HPLC.The analysis was performed on a RP-C18 column（4.6 mm × 250 mm

5 μm） with aceconitrile-0.1% phosphoric acid（26∶ 74） as mobile phase at a flow rate of 1.1 mL.min-1

and at room temperature.The detection of wave length was set at 326 nm.Result: The content of robinin-7-o-β-D-xylosylglucoside was 0.33%

and recovery rate was 98.62%.The content of total flavonoids was 1.90%

and recovery rate was 97.59%.Conclusion: The method is suitable for the determination of flavonoids components from D.mololariea.

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