GUO Yan-rong, FU Cui-li. Simultaneously Determination of Matrine and Stilbene Glucoside in Renzaozhiyang Capsule by SPE-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 75-77.
GUO Yan-rong, FU Cui-li. Simultaneously Determination of Matrine and Stilbene Glucoside in Renzaozhiyang Capsule by SPE-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 75-77. DOI: 10.13422/j.cnki.syfjx.2010.12.024.
固相萃取-HPLC同时测定润燥止痒胶囊中苦参碱和二苯乙烯苷的含量
摘要
目的:建立润燥止痒胶囊中苦参碱和二苯乙烯苷的含量测定方法。方法:采用固相萃取-HPLC
色谱柱为XTerraRP18(3.9 mm×150 mm
5μm)
流速1.0 mL·min-1
乙腈-水(20∶80)为流动相
检测波长为320 nm。结果:苦参碱的线性范围为0.302 6~1.513 g·L-1
r=0.999 7
平均回收率为98.65%
RSD 1.90%(n=6);二苯乙烯苷的线性范围为1.048~8.384 mg·L-1
r=0.999 5
平均回收率为97.45%
RSD 1.89%(n=6)。结论:方法简便、快速、准确
适合该胶囊中苦参碱和二苯乙烯苷的含量测定。
Abstract
Objective: To establish a method for the determination of matrine and stilbene glucoside in Renzao Zhiyang Capsule.Method: SPE-HPLC was conducted on a XTerra RP18 column(3.9 mm × 150 mm
5 μm) with acetonitrile-water(20∶80)
flow rate 1.0 mL·min-1
detecting at 320 nm.Result: Matrine was linear in the range of 0.302 6-1.513 g·L-1(r=0.999 7)
the average recovery was 98.65%
RSD 1.90%(n=6).Stilbene glucoside was linear in the range of 1.048-8.384 mg·L-1(r = 0.999 5)
the average recovery was 97.45%
RSD 1.89%(n=6).Conclusion: The method was simple
sensitive
accurate and suitable for the determination of matrine and stilbene glucoside in Renzao Zhiyang Capsule.