Objective: To investigate the effects of protein active composition of Scorpio on inhibition of proliferation and on apoptosis in Bel7402 cells

and to supply the data on establishing the quality evaluation method of biological effect guided by the clinical effect of Scorpio.Method: Bel7402 cells were examined by trypan blue exclusion.The proliferation of cells was determined by improving MTT assay.Flow cytometry was performed to analyze cell apoptosis with propidium iodide（PI）.Result: When the concentration of protein active composition of Scorpio exceeded or equaled 37 g.L-1

the coefficient correlation of the growth inhibiting curve to Bel7402 cells was 0.912 5

and IC50is 241 g.L-1.The excellence time was 0 to 48 hours.When the concentration of protein active composition of Scorpio exceeded or equaled 9.25 g.L-1

the apoptosis ratio of Bel7402 cells was raised significantly.Conclusion: The protein active composition of Scorpio might promote the apoptosis of Bel7402 cells and restrain the proliferation of them.The antitumor biological effect of the protein active composition of Scorpio was 9.25-175 g.L-1.The protein active composition may be one of the indexes for quality evaluation of biological effect of Scorpio.