Objective: To study the effects of Fuzheng Xiaozheng Formula（pharmaceutic serum） in mice with transplanted liver cancer（H22） on inhibiting angiogenesis and metastasis.Method: mice were randomly divided into 4 groups

to prepare 4 kinds of serums

including pharmaceutic serum of normal mice

control serum of normal mice

pharmaceutic serum of tumor-bearing mice

control serum of tumor-bearing mice.Model with transplanted liver cancer（H22） in mice was made by double-grafted tumor system.We injected the serum to each group on the 4

5

6 day except the normal group

and observed the tumors changes of each side and calculated the inhibitory of tumor（IR）.Concentration of IL-12 was determined by enzyme linked immunosorbent assay（ELISA）

immune histochemistry and graphical analysis were used to detect the expression of matrix metalloproteinase-9（MMP-9）of tumor tissues.Result: The serum of Fuzheng Xiaozheng Formula had positive effects in inhibiting the growth of the double flank tumor

it showed differences from the control serum group（P < 0.01）.The serum of tumor-bearing mice as no differences from the serum of normal mice.The group of Fuzheng Xiaozheng Formula had positive effects in raising the expression of IL-12 and inhibiting the expression of MMP-9 in tissue.It showed differences from the control serum group（P < 0.01）.Conclusion: One of mechanisms responsible for Fuzheng Xiaozheng Formula’s effect on angiogenesis and metastasis of tumor might be related to regulating the expression of IL-12 and inhibiting the expression of MMP-9.