芭蕉根HPLC指纹图谱鉴别研究

王祥培; 孙宜春; 靳凤云; 许士娜; 吴红梅; 黄婕

doi:10.13422/j.cnki.syfjx.2010.12.056

您当前的位置：

首页 >

文章列表页 >

芭蕉根HPLC指纹图谱鉴别研究

化学与分析 | 更新时间：2024-09-23

- 芭蕉根HPLC指纹图谱鉴别研究
- Identification of Musa basjoo by HPLC Fingerprint
- 中国实验方剂学杂志 2010年16卷第12期页码：30-33
- 作者机构：
  
  贵阳中医学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（30860387）;贵州省科学技术基金（J20072138）
- DOI：10.13422/j.cnki.syfjx.2010.12.056
  中图分类号： R284.1
- 纸质出版日期：2010
- 稿件说明：
移动端阅览
[1]王祥培,孙宜春,靳凤云,许士娜,吴红梅,黄婕.芭蕉根HPLC指纹图谱鉴别研究[J].中国实验方剂学杂志,2010,16(12):30-33.

WANG Xiang-pei, SUN Yi-chun, JIN Feng-yun, et al. Identification of Musa basjoo by HPLC Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 30-33.
[1]王祥培,孙宜春,靳凤云,许士娜,吴红梅,黄婕.芭蕉根HPLC指纹图谱鉴别研究[J].中国实验方剂学杂志,2010,16(12):30-33. DOI： 10.13422/j.cnki.syfjx.2010.12.056.

WANG Xiang-pei, SUN Yi-chun, JIN Feng-yun, et al. Identification of Musa basjoo by HPLC Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 30-33. DOI： 10.13422/j.cnki.syfjx.2010.12.056.

摘要

目的:建立芭蕉根的HPLC指纹图谱鉴别方法。方法:采用RP-HPLC-DAD法

以Diamonsil-C18（4.6 mm×250mm

5μm）色谱柱

流动相乙腈-0.05%磷酸水

采用梯度洗脱

流速1.0 mL.min-1

310 nm波长下测定芭蕉根、香蕉根及美人蕉根的指纹图谱

并作相似度比较分析。结果:建立了芭蕉根药材HPLC-UV指纹图谱

20个共有峰被标定

并对芭蕉根、香蕉根及美人蕉根药材的HPLC指纹图谱进行相似度比较

结果差异明显。结论:HPLC指纹图谱具有重复性好

特征性强

方法简便等特点

可用于芭蕉根药材的鉴别。

Abstract

Objective: To establish a HPLC fingerprint for the identification of Musa basjoo.Method: The analysis was carried out on a Diamonsil C18（4.6 mm × 250 mm

5 μm）with a mobile phase consisting of acetonitrile0.05% phosphoric acid

gradient eluent

the flow rate of 1.0 mL.min-1.The wave length was 310 nm

data were analyzed by fingerprint similarity evalution software to compare the similarity of samples.Result: The mutual mode to HPLC-UV fingerprints was estabilished

and the 20 mutual peaks were indicated.The similarities among the of Musa basjoo

Musa nana and Canna indica collected from different sources were obtained.There were apparent difference in fingerprint.Conclusion: HPLC fingerprint method is repeatable and feasible and can be suitable for identifying Musa basjoo.

关键词

Keywords

references

浏览量

220

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

菝葜药材HPLC指纹图谱的鉴别

不同基原石决明及其伪品的特征离子鉴别

砂仁的化学成分、药理活性及鉴别的研究进展