Objective: To establish UPLC fingerprint profile for the fast assessment on the similarity of Xiangdan injection.Method: Xiangdan injection was separated on an Acquity UPLC BEH C18 column.The mobile phase consisted of formic acid-water and acetonitrile was used for separation by gradient elution.The detection wavelength was set at 288 nm.The fingerprint profile was then established for analysis of Xiangdan injection from 9 corporations and 10 batches of one corporation on the base of the above chromatogarphy condition.It was pretreated by the methods such as baseline correction and peak matching and then the similarity of fingerprint profile was studied by the comparison of Xiangdan injection from different batches.Result: The fingerprint profile was able to reflect the message of quality change to some extent and it has little differences from the method of peak area.Conclusion: UPLC fingerprint profile can be used for the rapid assessment on the similarity of Xiangdan injection and it provided a good approach to evaluate rapidly the quality of traditional Chinese medicine and control the production process.