Objective:To develop an HPLC method for determination of Buddleoside from Flos Chrysanthemi Indici. The result was compared with IR spectrums. Method:Flos Chrysanthemi Indici from various habitats were used as test sample and HPLC was used. The chromatographic separation was performed on a Diamonsil C18（4. 6 mm × 250 mm

5 μm） column gradient eluted with a mobile phase of 0. 5% H3PO4（A）-acetonitrile（B）

B changed from 10% to 20% in the first 10 min

in the next 20 min

when B changed from 20% to 30%

and in the last 10 min

B changed to 40% . The detection wavelength was set at 326 nm and column temperature was set at 35 ℃ . The FTIR spectrums

the second-order derivative spectrogram and the two-dimensional infrared correlation spectrograms were used. Result:Buddleoside was linear within the range of 0. 262-5. 24 μg （r = 0. 999 91）. The average recovery was 98. 75%

with RSD 1. 02% （n = 5）. There were differences of samples on the second-order derivative spectrogram of Flos Chrysanthemi Indici. in 1 700-1 400 cm -1 and the two-dimensional infrared correlation spectrograms could be classified between frequency range 1 800-800 cm -1. Conclusion:The method was accurate

repetable and suitable to determine buddleoside from Flos Chrysanthemi Indici. The method can be used for quality control of Flos Chrysanthe- mi Indici. IR spectrum can also be used to identify the flavonoids from Flos Chrysanthemi Indici.