CHEN Feng-lian1, ZHANG Wen-jin2, XU Hong-hua1. HPLC Fingerprints of Overground part of Desmodium styracifolium in Different Collecting Time and Different Growing Areas[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 96-98.
CHEN Feng-lian1, ZHANG Wen-jin2, XU Hong-hua1. HPLC Fingerprints of Overground part of Desmodium styracifolium in Different Collecting Time and Different Growing Areas[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 96-98. DOI: 10.13422/j.cnki.syfjx.2010.14.048.
不同采收期及不同产地广金钱草地上部分HPLC指纹图谱研究
摘要
目的:建立广金钱草地上部分化学成分指纹图谱研究方法
对不同采收期及不同产地的广金钱草指纹图谱进行研究。方法:采用RP-HPLC法
DiamonsilTM C18色谱柱(迪马公司
4.6 mm×250 mm
5μm)
甲醇-0.2%磷酸水溶液(28∶72)
流速1 mL.min-1
检测波长271 nm
柱温40℃。结果:广东阳春产广金钱草与其他产地色谱指纹图谱差别较大
不同采收期药材黄酮成分含量有明显的差别。结论:该色谱条件简单易行
可用于广金钱草的鉴别研究。
Abstract
Objective:The HPLC fingerprints of chemical composition were established to evaluate different collecting time and different growing areas of overground part of Desmodium styracifolium.Method:HPLC analysis was performed on a DiamonsilTM C18column(4.6 mm × 250 mm
5 μm) with the mobile phase in isocratic elution :ethanol(A)-H2O(0.2% phosphoric acid)(28∶ 72).The column temperature was setup at 40 ℃ and the flow-rate was 1.0 mL.min-1.The detection of wavelength was at 271 nm.Result:The D.styracifolium from Yangchun has distinct differences with other place of origin;The accumulation of flavones constituents showed a regular pattern in different collecting time.Conclusoin:The method is reliable and can be helpful on the quality control of D.styraci-folium.