Objective:To observe the Effects of Suanzaoren Tang（SZRT） on glutamic acid（Glu）

γ-aminobutyric acid（GABA） and γ-aminobutyric acid subtype A rceptor（GABAAR） expression in the brain of the elderly insomnia model in rats.Method:Multiple factors model were established with subacute senescence induced by D-galactose

Yin and blood-deficiency induced by cyclophosphamide and hydrocortisonum

and sleep deprivation（SD） induced by a modified multiple platform method（MMPM）.The levels of amino acid neurotransmitters Glu and GABA were detected in cortex and hypothalamus for the rats by HPLC using immunohistochemical staining and semi-quantitative analysis of a retrovirus-polymerase chain reaction（RT-PCR）

subunit expression of GABAAR α1 and γ2 were detected in cortical and hippocampal parts of the model rats.After intervention of SZRT

thoseindicators were investigated too.Result:Compared with the environmental control group

content of Glu

GABA and the ratio of them in cortex and hypothalamus in the insomnia model rats showed a considerable elevatio

GABAARα1 and γ2 subunits expressed by immunochemical accumulated absorbance in cortex and hippocampus in the models were significantly higher（P < 0.01）

and GABAARα1

γ2 mRNA expression in cortex showed higher significantly（P < 0.01）.While these indicators tended to be in the normal range after the treatment of SZRT.Conclusion:Markedly up-regulated expression of GABAAR α1 and γ2 in cerebral cortex and hippocampus may be one of the pathological mechanisms for the insomnia model with sleep deprivation.SZRT can reduce the toxic effects of amino acids in brain

and downward adjust GABAAR α1 and γ2subunit expression in the brain.This may be one of the mechanisms for SZRT treatment of insomnia.