3种不同治法方药对大鼠心肌缺血再灌注损伤核因子-кB信号转导通路的影响

唐丹丽; 刘寨华; 隋宇; 佟林; 张华敏

doi:10.13422/j.cnki.syfjx.2010.14.064

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3种不同治法方药对大鼠心肌缺血再灌注损伤核因子-кB信号转导通路的影响

药理 | 更新时间：2024-09-23

- 3种不同治法方药对大鼠心肌缺血再灌注损伤核因子-кB信号转导通路的影响
- Comparative Studies on Effects of Three Different Chinese Medicine Treatments on NF-кB Signal Transduction Pathway of Myocardial Ischemia-reperfusion Injury in Rats
- 中国实验方剂学杂志 2010年16卷第14期页码：161-164
- 作者机构：
  
  1. 中国中医科学院医学实验中心
  2. 中国中医科学院中医基础理论研究所
  3. 中国中医科学院中医药信息研究所
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（30701066）
- DOI：10.13422/j.cnki.syfjx.2010.14.064
  中图分类号： R285.5
- 纸质出版日期：2010
- 稿件说明：
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[1]唐丹丽,刘寨华,隋宇,佟林,张华敏.3种不同治法方药对大鼠心肌缺血再灌注损伤核因子-кB信号转导通路的影响[J].中国实验方剂学杂志,2010,16(14):161-164.

TANG Dan-li1, LIU Zhai-hua2, SUI Yu1, et al. Comparative Studies on Effects of Three Different Chinese Medicine Treatments on NF-кB Signal Transduction Pathway of Myocardial Ischemia-reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 161-164.
[1]唐丹丽,刘寨华,隋宇,佟林,张华敏.3种不同治法方药对大鼠心肌缺血再灌注损伤核因子-кB信号转导通路的影响[J].中国实验方剂学杂志,2010,16(14):161-164. DOI： 10.13422/j.cnki.syfjx.2010.14.064.

TANG Dan-li1, LIU Zhai-hua2, SUI Yu1, et al. Comparative Studies on Effects of Three Different Chinese Medicine Treatments on NF-кB Signal Transduction Pathway of Myocardial Ischemia-reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 161-164. DOI： 10.13422/j.cnki.syfjx.2010.14.064.

摘要

目的:观察中医不同治法方药对大鼠心肌缺血再灌注损伤（I/R）核因子-кB（NF-кB）信号转导通路的影响。方法:将60只SD大鼠随机分为5组:假手术组、模型组、祛痰宽胸组、活血化瘀组及痰瘀同治组。可逆性冠脉左前降支结扎缺血30 min再灌注2 h复制I/R模型

应用Western blotting及RT-PCR法检测各组大鼠心肌核因子IкB诱导激酶（NIK）、IкB激酶β（IKKβ）、核转录因子抑制蛋白（IкBα）及NF-кBp65 mRNA的表达。结果:与模型组比较

各治疗组均能降低心肌NIK

IKKβ

NF-кBp65 mRNA水平并上调IкBα蛋白表达（P<0.01）。结论:祛痰宽胸法、活血化瘀法及痰瘀同治法均对I/R大鼠有保护作用

其主要作用机制可能是通过抑制NIK

IKKβ蛋白表达

减少IкBα蛋白的磷酸化降解而增加其蛋白水平

从而抑制NF-кB的过度活化

发挥其对缺血再灌注损伤心肌的保护作用。

Abstract

Objective:To investigate the influence of different Chinese medicine treatments on NF-кB signal transduction pathway of myocardial ischemia-reperfusion（I /R） injury in rats.Method:Sixty SD rats were divided into 5 groups randomly

sham-operated group:model group

removing phlegm and enlarging chest group

activating blood and dissolving stasis group

treating both phlegm and blood stasis group.The model of I /R of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats.NIK

IKKβ and IкBα protein expression of myocardial tissues in rats were detected by Western blotting method.And the levels of NF-кBp65 mRNA were determined with RT-PCR.Result:Compared with model group

the expression of NIK

IKKβ and NF-кBp65 mRNA were lower

and the protein expression of IкBα was increased in all groups treated by different Chinese medicine treatments（P < 0.01）.Conclusion:The removing phlegm andenlarging chest method

activating blood and dissolving stasis method

treating both phlegm and blood stasis method can protect myocardium from I /R.The mechanism of action was related to inhibiting the protein expression of NIK

IKKβ and enhancing the expression of IкBα

restraining the activation of NF-кB.

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