健脾解毒方介导p38MAPK信号转导下调幽门螺杆菌诱导的胃癌细胞环氧合酶2启动子活性

王炎; 刘宁宁; 周利红; 吴琼; 周宁; 孙珏; 范忠泽; 李琦

doi:10.13422/j.cnki.syfjx.2010.14.073

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健脾解毒方介导p38MAPK信号转导下调幽门螺杆菌诱导的胃癌细胞环氧合酶2启动子活性

药理 | 更新时间：2024-09-23

- 健脾解毒方介导p38MAPK信号转导下调幽门螺杆菌诱导的胃癌细胞环氧合酶2启动子活性
- Jianpi Jiedu Recipe Intervenes Signal Transduction of p38 Mitogen-activated Protein kinases and Inhibits Activity of Cyclooxygenase-2 Promoter Induced by Helicobacter pylori in Gastric Cancer Cell MKN45
- 中国实验方剂学杂志 2010年16卷第14期页码：105-109
- 作者机构：
  
  1. 上海中医药大学附属普陀医院实验中心
  2. 上海中医药大学附属普陀医院肿瘤科
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（30600844）,（81072955）;上海市普陀区科委科技创新重大项目（2009-37-2）;上海市重点学科资助项目（S30302）;上海市教委青年基金（09JW44）
- DOI：10.13422/j.cnki.syfjx.2010.14.073
  中图分类号： R735.2;R285.5
- 纸质出版日期：2010
- 稿件说明：
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[1]王炎,刘宁宁,周利红,吴琼,周宁,孙珏,范忠泽,李琦.健脾解毒方介导p38MAPK信号转导下调幽门螺杆菌诱导的胃癌细胞环氧合酶2启动子活性[J].中国实验方剂学杂志,2010,16(14):105-109.

WANG Yan1, LIU Ning-ning1, ZHOU Li-hong1, et al. Jianpi Jiedu Recipe Intervenes Signal Transduction of p38 Mitogen-activated Protein kinases and Inhibits Activity of Cyclooxygenase-2 Promoter Induced by Helicobacter pylori in Gastric Cancer Cell MKN45[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 105-109.
[1]王炎,刘宁宁,周利红,吴琼,周宁,孙珏,范忠泽,李琦.健脾解毒方介导p38MAPK信号转导下调幽门螺杆菌诱导的胃癌细胞环氧合酶2启动子活性[J].中国实验方剂学杂志,2010,16(14):105-109. DOI： 10.13422/j.cnki.syfjx.2010.14.073.

WANG Yan1, LIU Ning-ning1, ZHOU Li-hong1, et al. Jianpi Jiedu Recipe Intervenes Signal Transduction of p38 Mitogen-activated Protein kinases and Inhibits Activity of Cyclooxygenase-2 Promoter Induced by Helicobacter pylori in Gastric Cancer Cell MKN45[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 105-109. DOI： 10.13422/j.cnki.syfjx.2010.14.073.

摘要

目的:探讨幽门螺杆菌（Helicobacter pylori）对人胃癌MKN45细胞环氧合酶2（Cyclooxygenase-2

COX-2）启动子荧光素酶报告基因重组质粒（pGL3-Basic-COX-2-promoter）活性的影响和健脾解毒方对其的调控作用及可能的机制。方法:将构建的pGL3-Basic-COX-2-promoter转染MKN45细胞

观察H.pylori对其活性的影响。运用p38MAPK特异性抑制剂SB203580阻断p38MAPK信号通路

观察H.pylori对MKN45细胞COX-2启动子活性的影响。Western blot法检测健脾解毒方对H.pylori感染的MKN45细胞p38MAPK信号通路及其下游激活转录因子-2（ATF-2）表达的影响。结果:H.pylori可增加COX-2启动子活性;抑制p38MAPK信号通路后

COX-2启动子活性明显下调;健脾解毒方能够抑制H.pylori诱导的p38MAPK及ATF-)的活性。结论:H.pylori通过p38MAPK信号通路上调胃癌细胞COX-2启动子活性;健脾解毒方通过调控p38MAPK/ATF-2信号通路

抑制COX-2启动子活性

是其防治H.pylori诱发胃癌的机制之一。

Abstract

Objective:To investigate the effect of Helicobacter pylori on pGL3-Basic-COX-2-promoter transcriptional activity in gastric cancer MKN45 cells and the regulatory mechanism of Jianpi Jiedu Recipe on COX-2 promoter transcriptional activity.Method:The recombinant vector pGL3-Basic-COX-2-promoter was transient co-transfected into MKN45 cells

then detected the effect of H.pylori on COX-2 promoter activity.The effect of H.pylori on COX-2 promoter activity in human gastric cancer cells after blocking p38MAPK signal transduction pathway with a specific inhibitor SB203580 was observed.The effect of Jianpi Jiedu Recipe on H.pylori-stimulated phosphorylation of p38MAPK and ATF-2

the downstream transcription factor of p38MAPK

was investigated.Result:H.pylori could increase COX-2 promoter activity.COX-2 promoter activity down-regulated significantly afterblocking p38MAPK signal transduction pathway.Jianpi Jiedu Recipe inhibited H.pylori-induced p38MAPK and ATF-2 activity.Conclusion:H.pylori infection increases COX-2 promoter transcriptional activity via p38MAPK signal transduction pathway

and Jianpi Jiedu Recipe inhibits H.pylori-induced COX-2 promoter transcriptional activity through regulating p38MAPK/ATF-2 signal transduction pathway

which may be one of the mechanisms of prevention and treatment of gastric cancer.

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