金银花叶中绿原酸的大孔吸附树脂纯化工艺研究

杨军宣; 尹蓉莉; 陈金玉; 吴天骄; 王宝才; 陈海亭

doi:10.13422/j.cnki.syfjx.2010.15.008

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金银花叶中绿原酸的大孔吸附树脂纯化工艺研究

工艺与制剂 | 更新时间：2024-09-23

- 金银花叶中绿原酸的大孔吸附树脂纯化工艺研究
- Study on Purification of Chlorogenic Acid from Lonicera Japonica Leaves with Macroporous Resin
- 中国实验方剂学杂志 2010年16卷第15期页码：20-23
- 作者机构：
  
  1. 成都中医药大学药学院
  2. 四川迪康科技药业股份有限公司
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.15.008
  中图分类号： R284.1
- 纸质出版日期：2010
- 稿件说明：
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[1]杨军宣,尹蓉莉,陈金玉,吴天骄,王宝才,陈海亭.金银花叶中绿原酸的大孔吸附树脂纯化工艺研究[J].中国实验方剂学杂志,2010,16(15):20-23.

YANG Jun-xuan1, YIN Rong-li1, CHEN Jin-yu1, et al. Study on Purification of Chlorogenic Acid from Lonicera Japonica Leaves with Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 20-23.
[1]杨军宣,尹蓉莉,陈金玉,吴天骄,王宝才,陈海亭.金银花叶中绿原酸的大孔吸附树脂纯化工艺研究[J].中国实验方剂学杂志,2010,16(15):20-23. DOI： 10.13422/j.cnki.syfjx.2010.15.008.

YANG Jun-xuan1, YIN Rong-li1, CHEN Jin-yu1, et al. Study on Purification of Chlorogenic Acid from Lonicera Japonica Leaves with Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 20-23. DOI： 10.13422/j.cnki.syfjx.2010.15.008.

摘要

目的:建立金银花叶中绿原酸的含量测定方法

考察不同型号的大孔吸附树脂对金银花叶中绿原酸的提取纯化效果

为金银花药材的综合开发利用提供参考。方法:采用高效液相色谱法测定指标成分。采用静态吸附法和动态吸附法

考察大孔树脂的吸附、洗脱附性能和纯化效果。结果:绿原酸在2.376 8～60.476 9μg与峰面积呈良好线性关系

平均加样回收率96.82%

RSD 1.83%。HPD450大孔吸附树脂纯化效果较好

优化条件为:柱径比3∶1

药液质量浓度0.5～1.0 g·mL-1、吸附速度0.5 BV·h-1、树脂吸附量生药0.3 g·g-1、洗脱溶媒30%乙醇、洗脱速度2 B V·h-1

洗脱溶媒用量2 BV。结论:HPD450大孔树脂对绿原酸纯化效果较好

工艺稳定可行

可用于工业化生产。

Abstract

Objective:To establish a method for determination of chlorogenic acid in Lonicera japonica leaves by HPLC

and study on extracting and purifying chlorogenic acid from Lonicera japonica leaves by different macroporous resin

so as to provide a reference for the comprehensive development and utilization of honeysuckle herbs.Method: The chlorogenic acid was determined with the detection wavelength 327 nm by HPLC.Static adsorption and dynamic adsorption were used to optimize the adsorption and elution process by macroporous resin.Result:Chlorogenic acid was linear in the range of 2.376 8-60.476 9 μg.The average recovery was 96.82%

RSD was 1.83%.At the same time

the purification effect of chlorogenic acid by HPD450 macroporous resin was better than other resins

the optimal conditions were:column diameter ratio 3 ∶ 1

liquid concentration 0.5-1.0 g·mL-1

absorption velocity 0.5 BV·h-1

resin adsorption quantity 0.3 g·g-1

elution solvent 30% ethanol

elution speed 2 BV·h-1

elution solvent volume 2 BV.Conclusion:The process of purification of chlorogenic acid from Lonicera japonica leaves by HPD450 macroporous resin was stable and could be used for industrial production.

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