HPLC同时测定丹参水溶性及脂溶性5种成分的含量

张慧娴; 杜守颖; 陆洋; 陈晓兰; 宋逍; 李冬雪; 徐冰

doi:10.13422/j.cnki.syfjx.2010.15.020

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HPLC同时测定丹参水溶性及脂溶性5种成分的含量

化学与分析 | 更新时间：2024-09-23

- HPLC同时测定丹参水溶性及脂溶性5种成分的含量
- Simultaneous Determination of Five Hydrophilic and Lipophilic Active Components in Radix et Rhizoma Salviae Miltiorrhizae by HPLC
- 中国实验方剂学杂志 2010年16卷第15期页码：34-38
- 作者机构：
  
  1. 北京中医药大学中药学院
  2. 中国生物技术发展中心
- 作者简介：
- 基金信息：
  
  “重大新药创制”科技重大专项（2009ZX09502-008）;“中药生产技术及过程控制技术标准平台”（2009ZX09308-003）;教育部博士点基金（20090013110007）
- DOI：10.13422/j.cnki.syfjx.2010.15.020
  中图分类号： R284.1
- 纸质出版日期：2010
- 稿件说明：
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[1]张慧娴,杜守颖,陆洋,陈晓兰,宋逍,李冬雪,徐冰.HPLC同时测定丹参水溶性及脂溶性5种成分的含量[J].中国实验方剂学杂志,2010,16(15):34-38.

ZHANG Hui-xian1, DU Shou-ying1, LU Yang1, et al. Simultaneous Determination of Five Hydrophilic and Lipophilic Active Components in Radix et Rhizoma Salviae Miltiorrhizae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 34-38.
[1]张慧娴,杜守颖,陆洋,陈晓兰,宋逍,李冬雪,徐冰.HPLC同时测定丹参水溶性及脂溶性5种成分的含量[J].中国实验方剂学杂志,2010,16(15):34-38. DOI： 10.13422/j.cnki.syfjx.2010.15.020.

ZHANG Hui-xian1, DU Shou-ying1, LU Yang1, et al. Simultaneous Determination of Five Hydrophilic and Lipophilic Active Components in Radix et Rhizoma Salviae Miltiorrhizae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 34-38. DOI： 10.13422/j.cnki.syfjx.2010.15.020.

摘要

目的:建立同时测定丹参中2种水溶性和3种脂溶性成分的HPLC方法。方法:采用C18柱（4.6 mm×250 mm

5μm）

以乙腈（A）-0.05%磷酸水溶液（B）为流动相进行梯度洗脱:0～27 min

20%～25%A;27～55 min

25%～80%A;55～65min

80%～80%A;检测波长为270 nm和286 nm

柱温30℃。结果:迷迭香酸、丹酚酸B、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA分别在1.92×10-2～0.96μg

7.29×10-2～3.64μg

3.05×10-3～0.15μg

1.71×10-3～8.55×10-2μg

5.80×10-3～0.29μg呈良好的线性关系。加样回收率分别为99.88%

104.75%

101.08%

101.41%

102.41%。结论:该含量测定方法准确、稳定、简便

分离效果好

能同时测定丹参中迷迭香酸、丹酚酸B、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA 5种有效成分的含量。

Abstract

Objective: To develop a new method for simultaneous determination of two hydrophillc components and three lipophillc components of Radix et Rhizoma Salviae Miltiorrhizae.Method:The HPLC-double wavelength detection was employed using a column of Dikma Diamonsil C18（4.6 mm × 250 mm

5 μm） with a mobile phase of acetonitrile（A）-0.05% phosphoric acid（B）.The gradient elution program was as follows:0-27 min

20%-25% A;27-55 min

25%-80% A;55-65 min

80%-80% A.The detection wavelength was set at 270 nm and 286 nm.The column temperature was at 30 ℃.Result:Rosmarinic acid was linear in the range of 1.92 × 10-2-0.96 μg;salviamolic acid B was linear in the range of 7.29 × 10-2-3.64 μg;clyptotanshinon was linear in the range of 3.05 × 10-3-0.15 μg;tanshinone was linear in the range of 1.71 × 10-3-8.55 × 10-2 μg and tanshinone ⅡA was linear in the range of 5.80 × 10-3-0.29 μg.The average recovery was 99.88%

104.75%

101.08%

101.41%

102.41% respectively.Conclusion:The present method was simple and had satisfactory efficacy;it can simultaneously determine multiple hydrophilic and lipophilic bioactive components in Salvia miltiorrhiza from different areas.

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