Objective:To investig

ate the therapeutic effect of Anzi Mixture on concentration of positive serum anticardiolipid antibody（ACA） in cyclophosphamide-treated mice.Method:Cyclophosphamide was given to induce positive serum ACA for the model mice.Control group was given saline（0.01 mL.g-1） and distilled water（0.01 mL·g-1）;model group was received cyclophosphamide（0.01 mL·g-1）and distilled water（0.01 mL·g-1）.The treatment groups were given aspirin or high

middle

low doses of Anzi Mixture（0.01 mL·g-1） on the model of cyclophosphamide induced.All groups were administered once daily for 14 consecutive days.Eyeball blood sample was collected for all the mice in all groups 1 h after administration on the 14 th day

and then serum was separated by centrifugation

ELISA was employed to detect the serum ACA levels.Result:Compared with the model group

sig-nificant decrease in serum ACA level was observed in all groups of different dosages of Anzii Mixture（P < 0.05）.Compared to aspirin group

there was no obvious difference in serum ACA level for any group of Anzi Mixture.Con-clusion:Anzi Mixture could effectively reduce serum ACA level in cyclophosphamide-treated mice with a dose-de-pendent manner.The potency of Anzi Mixture treatment appeared to be equal to that of aspirin treatment.