ZHOU Nong, GUO Ji-fen, YANG Li-yun, et al. Determination of Diosgenin in Paris polyphylla var. yunnanensis at Different Collecting Time by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 54-56.
ZHOU Nong, GUO Ji-fen, YANG Li-yun, et al. Determination of Diosgenin in Paris polyphylla var. yunnanensis at Different Collecting Time by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 54-56. DOI: 10.13422/j.cnki.syfjx.2010.18.030.
HPLC测定不同采收期滇重楼中薯蓣皂苷元的含量
摘要
目的:通过测定不同采收期滇重楼中薯蓣皂苷元的含量
为确定滇重楼的采收期提供参考依据。方法:采用高效液相色谱法
色谱柱为Agilent Zorbax SB-C18(4.6 mm×150 mm
5μm)
流动相为乙腈-水(90∶10)
流速为1.0 mL.min-1
检测波长203 nm
柱温35℃。结果:不同采收期滇重楼中薯蓣皂苷元含量发生明显变化。该成分在4-6月含量逐渐升高
5-6月含量最高
6-7月含量骤降且降幅较大
7-10月含量基本上一致。结论:滇重楼不同采收期薯蓣皂苷元的含量是不同
在实际应用时根据不同需要适时采收。
Abstract
Objective:To determine diosgenin in Paris polyphylla var.yunnanensis at different collecting time.Method:Diosgenin was separated on a Zorbax SB-C18(4.6 mm × 150 mm
5 μm) column and detected at 203 nm at the flow rate of 1.0 mL.min-1 using acetonitrile-water(90∶ 10) as the mobile phase.Column tempera-ture was at 35 ℃.Result: There were differences in the contents of diosgenin in P.polyphylla var.yunnanensis at different collecting time.The contents of diosgenin increased from April to June and reached the highest level in May and June.However
it decreased sharply in June and July.The contents maintained the same level from July to October.Conclusion: The contents of diosgenin in P.polyphylla var.yunnanensis varied with the collecting seasons.