含齐墩果酸和熊果酸的10种果实类中药HPLC分析

査孝柱; 谢晓梅; 吕美红; 周安; 刘先华

doi:10.13422/j.cnki.syfjx.2010.18.038

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含齐墩果酸和熊果酸的10种果实类中药HPLC分析

化学与分析 | 更新时间：2024-09-23

- 含齐墩果酸和熊果酸的10种果实类中药HPLC分析
- Analysis of Fruits from Ten Chinese Medicinal Herbs Containing Oleanolic Acid and Ursolic Acid by HPLC
- 中国实验方剂学杂志 2010年16卷第18期页码：60-62
- 作者机构：
  
  安徽中医学院现代中药安徽省重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.18.038
  中图分类号： R284.1
- 纸质出版日期：2010
- 稿件说明：
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[1]査孝柱,谢晓梅,吕美红,周安,刘先华.含齐墩果酸和熊果酸的10种果实类中药HPLC分析[J].中国实验方剂学杂志,2010,16(18):60-62.

ZHA Xiao-zhu, XIE Xiao-mei, LV Mei-hong, et al. Analysis of Fruits from Ten Chinese Medicinal Herbs Containing Oleanolic Acid and Ursolic Acid by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 60-62.
[1]査孝柱,谢晓梅,吕美红,周安,刘先华.含齐墩果酸和熊果酸的10种果实类中药HPLC分析[J].中国实验方剂学杂志,2010,16(18):60-62. DOI： 10.13422/j.cnki.syfjx.2010.18.038.

ZHA Xiao-zhu, XIE Xiao-mei, LV Mei-hong, et al. Analysis of Fruits from Ten Chinese Medicinal Herbs Containing Oleanolic Acid and Ursolic Acid by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 60-62. DOI： 10.13422/j.cnki.syfjx.2010.18.038.

摘要

目的:建立高效液相色谱法测定10种果实类中药材:山茱萸、山楂、女贞子、木瓜、乌梅、连翘、石榴皮、柿蒂、夏枯草以及榠楂中齐墩果酸与熊果酸的含量

并进行色谱图比较

探讨其鉴别特征信息。方法:使用Shim-pack CLC-ODS色谱柱（4.6 mm×250 mm

5μm）

流动相为甲醇-水-冰醋酸-三乙胺（265∶35∶0.1∶0.05）

流速0.6 mL.min-1

二极管阵列检测器

检测波长为210 nm。结果:连翘、夏枯草和女贞子齐墩果酸、熊果酸总含量较高;10种中药材在所建立方法的检测和分析中表现出一定的鉴别特征。结论:该法简便、准确、重现性好

可用于这些含有齐墩果酸与熊果酸的果实类中药材的含量测定

HPLC的谱图信息分析也为该10种中药材鉴定提供了初步的实验资料。

Abstract

Objective:To establish an HPLC method for simultaneous determination of oleanolic acid and ur-solic acid from ten fruits of Chinese medicinal herbs

including Fructus Corni

Fructus Crataegi

Fructus Ligustri

Fructus Chaenomelis

Fructus Mume

Fructus Forsythiae

Pericarpium Granati

Calyx Kaki

Spica Prunellae and Fructus Chaenomelis Sinensis

and their chromatograms were compared.Method:Oleanolic acid and ursolic acid were separated on Shim-pack CLC-ODS（M）（4.6 mm × 250 mm

5 μm） with mobile phase consisted of methanol-water-glacial acetic acid-triethylamine（265∶ 35∶ 0.1∶ 0.05） at a flow rate of 0.6 mL.min-1.The detective wavelength was set at 210 nm by photodiode array detector（PDAD）.Result: The total contents of oleanolic acid and ursolic acid from Fructus Forsythiae

Spica Prunellae and Fructus Ligustri were higher than that of others.Some identification characteristics were shown in the analysis of ten kinds of Chinese herbal medicines under the conditions in this paper.Conclusion: This method was simple

accurate and reproducible

and suitable for the analysis of these drugs.The chromatogram information may be useful for the identification of the ten fruits from Chinese medicinal herbs.

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