REN Jie, LIU Guang-bin, ZOU Gan-peng, et al. Determination of Matrine in Shuyin Lotion by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 76-77.
REN Jie, LIU Guang-bin, ZOU Gan-peng, et al. Determination of Matrine in Shuyin Lotion by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 76-77. DOI: 10.13422/j.cnki.syfjx.2010.18.043.
HPLC测定舒阴洗液中苦参碱的含量
摘要
目的:建立舒阴洗液中苦参碱的含量测定方法。方法:采用高效液相色谱法
Inertsil ODS-SP色谱柱(4.6 mm×150 mm
5μm)
流动相乙腈-水-三乙胺(3∶97∶0.2
用磷酸调pH 3.5)
流速1.0 mL.min-1
检测波长220 nm。结果:苦参碱在3.9~117.0μg呈良好的线性关系(r=0.999 9)
平均回收率为101.69%
RSD 1.84%(n=9)。结论:本法简便、快速、准确
可用于该制剂的质量控制。
Abstract
Objective:To establish a method for determination of matrine in Shuyin Lotion.Method:The matrine content was determined by HPLC on inertsil ODS-SP column(4.6 mm × 150 mm
5 μm) with a mobile phase of acetonitrile-water-triethylamine(3∶ 97∶ 0.2
adjusted to pH 3.5 with phosphoric acid)
the flow rate was 1.0 mL.min-1
and the detection wavelength was set at 220 nm.Result: Matrine was linear in range of 3.9-117.0 μg(r =0.999 9).The average recovery was 101.69% with RSD of 1.84%(n =9).Conclusion: The method is simple
quick and accurate.It can be used for the quality control of Shuyin lotion.