Objective:To prepare the total glycosides of Paeonia lactiflora

and investigate its protection towards carbon tetrachloride induced acute liver injury and DL-ethionine induced acute fatty liver in mice.Method: Macroporous adsorption chromatography was used to enrich its total glycosides.Carbon tetrachloride induced acute liver injury and DL-ethionine induced acute fatty liver in mice were utilized to investigate the effect of total glycosides of P.lactiflora on serum alanine aminotransferase（ALT） and aspartate aminotransferase（AST） activities.Meanwhile

its effect on total cholesterol（TC） and triglyceride（TG） contents in liver was studied in DL-ethionine induced acute fatty liver model.Result: The polysaccharide and some other components was eluted off from the column of AB-8 resins with 10% ethanol

afterwards the remaining total glycosides was eluted off from the column by washing continuously with 50% ethanol

and its content was above 50%.On carbon tetrachloride induced acute liver injury model

the total glycosides could significantly reduce the increase of serum ALT and AST activities induced by CCl4;on DL-ethionine induced acute fatty liver model

there was no significant difference of serum ALT and AST activities between the model group and the control group

however

the content of total cholesterol and triglyceride in the liver of model groups significantly increased and decreased after treatment with total glycosides.Conclusion: The total glycosides of P.lactiflora has a protective effect on CCl4 induced acute liver injury and DL-ethionine induced acute fatty liver in mice.