白花蛇舌草总黄酮的大孔树脂纯化工艺

曾永长; 梁少瑜; 邢学峰; 俞励平; 罗佳波

doi:10.13422/j.cnki.syfjx.2010.18.059

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白花蛇舌草总黄酮的大孔树脂纯化工艺

工艺与制剂 | 更新时间：2024-09-23

- 白花蛇舌草总黄酮的大孔树脂纯化工艺
- Purification Process of Total Flavonoids from Hedyotis diffusa with Macroporous Resins
- 中国实验方剂学杂志 2010年16卷第18期页码：26-29
- 作者机构：
  
  南方医科大学中药新药重点实验室广东省中药制剂重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.18.059
  中图分类号： R284
- 纸质出版日期：2010
- 稿件说明：
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[1]曾永长,梁少瑜,邢学峰,俞励平,罗佳波.白花蛇舌草总黄酮的大孔树脂纯化工艺[J].中国实验方剂学杂志,2010,16(18):26-29.

ZENG Yong-chang, LIANG Shao-yu, YU Li-ping, et al. Purification Process of Total Flavonoids from Hedyotis diffusa with Macroporous Resins[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 26-29.
[1]曾永长,梁少瑜,邢学峰,俞励平,罗佳波.白花蛇舌草总黄酮的大孔树脂纯化工艺[J].中国实验方剂学杂志,2010,16(18):26-29. DOI： 10.13422/j.cnki.syfjx.2010.18.059.

ZENG Yong-chang, LIANG Shao-yu, YU Li-ping, et al. Purification Process of Total Flavonoids from Hedyotis diffusa with Macroporous Resins[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 26-29. DOI： 10.13422/j.cnki.syfjx.2010.18.059.

摘要

目的:为提高白花蛇舌草（HD）总黄酮的纯度

筛选适宜HD总黄酮纯化的大孔树脂

确定HD总黄酮纯化工艺参数。方法:采用静态与动态吸附-解吸附方法对HD总黄酮进行纯化

以吸附率与洗脱率等指标优化工艺

利用紫外-可见分光光度计测量HD总黄酮的含量。结果:HPD-826型大孔树脂纯化效果最好

其最佳工艺为上样流速2 BV.h-1

上样液pH 5

上样浓度1.5 g.L-1

洗脱剂pH 7

乙醇浓度80%与洗脱速度2 BV.h-1;纯化后总黄酮含量高达91.24%。结论:该工艺操作简单、稳定可行

重复性好

适合HD总黄酮纯化。

Abstract

Objective:Purify the total flavonoids from Hedyotis diffusa.with macroporous resin and establish optimum process for separating and purifying total flavonoids from H.diffusa.Method:The optimal techniques of separation and purification of flavonoid were obtained by static adsorption and elution tests

and the separation tech-niques were evaluated by measuring the concentration of total flavonoids from H.diffusa with ultraviolet spectropho-tometer.Result: The HPD-826 macroporous resin gave the best effect of separation with the sampling rate 2 BV.h-1

concentration 1.0 g.L-1

pH 5 respectively

and the concentration of elution was 80% alcohol

eluting veloc-ity 2 BV.h-1

with pH 7.Conclusion: HPD-826 resin could be applied to purify total flavonoids from H.diffusa.

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