Objective: To investigate the regulatory effects of Jianpi Jiedu Recipe on microvessel density（MVD） and angiogenesis-related genes in the course of Helicobacter pylori（H.pylori） long-term infection induced gastric adenocarcinoma of C57BL /6 mice

and provide experimental basis for Jianpi Jiedu Recioe treatment of the H.pylori-related gastropathy.Method: C57BL /6 mouse gastric cancer model induced by H.pylori infected was established by gavage with H.pylori standard strain SS1

and mice were divided into 4 groups: Control（Cont） group-model（Mod）group and low-dose（LD）and high-dose（HD）Jianpi Jiedu Recioe group.After 72 weeks

MVD in gastric mucosa of each group was detected with Evision immunohistochemical method.Vascular endothelial growth factor（VEGF）

angiopoietin-2（Ang-2） and Ang-2 type 2 receptor（Tie-2） mRNA and protein expressionwere evaluated by real-time fluorogenic quantitative polymerase chain reaction（RFQ-PCR） and immunohistochemical method.Result: Pathological examination demonstrated that at the 72 th week

the mice gastric mucosa MVD were 2.50 ± 1.54

18.56 ± 2.62

14.61 ± 3.60 and 7.39 ± 1.75 in Cont

Mod

LD and HD group respectively.The MVD in Mod group was obviously increased than that in Cont group

P < 0.01;The MVD were significantly decreased in LD and HD Jianpi Jiedu Recioe group

compared with Mod group

P < 0.05

P < 0.01.FRQ-PCR suggested that the expression of VEGF

Ang-2

Tie-2 mRNA and protein in Mod group were obviously increased than those in Cont group

P < 0.01;However both the LD and HD Jianpi Jiedu Recioe could significantly reduce their expression in a dose-dependent manner.Conclusion: H.pylori could increase MVD of gastric mucosa

and induce VEGF

Ang-2 and Tie-2 expression in the course of H.pylori infection-induced gastric adenocarcinoma of C57BL /6 mice

which might play an important role in the incidence of H.pylori-induced gastric cancer.Jianpi Jiedu Recipe could decrease MVD

and inhibit VEGF

Ang-2 and Tie-2 expression

which might be one of the important mechanisms of prevention and treatment of gastric cancer.