Objective:To consummate the quality standard and provide theory evidence of Begonia herbs

by constituting the method of assaying of rutin in Begonia herbs.Method: The content of rutin of Begonia L.herbs were determined by HPLC.Diamonsil Chromatographic column（Diamond）-C18（4.6 mm × 250 mm

5 μm）.Mobile phase was methanol-0.5% phosphoric acid gradient elution.Column temperature was 30 ℃.The detection wavelength 257 nm.Result: The content of rutin was influenced by different species

different origins and different medicinal parts

and the rutin content of leaf

stem which was higher than root.Among them

The content of rutin was the highest than others

which of Begonia cavalerei in Kaiyang Nanjiang canyou of Guizhou.Conclusion: The method by constituting is simple and convenient

reproducible

accurate.It can conduct method of assaying of rutin in Begonia herbs.It is right to collect the leaf and stem part of the herbs which provide a reliable basis for conservation and reasonable development of Begonia herbs resources in Guizhou.