Objective:To establish a method for studing HPLC chromatographic fingerprint of Polygonum cuspidate

and to control the quality of P.cuspidate.Method:The DIKMA-C18（4.6 mm × 200 mm

5 μm） column was used.The mobile phase was acetonitrile-0.1% phosphoric acid of water solution with gradient elution of linear

and the flow rate was 1.0 mL.min-1.The column temperature was 30 ℃

and the detection wavelength was set at 303 nm.Result: Thirteen batches samples of P.cuspidate were analyzed and the chromatographic fingerprint was constructed with 13 common fingerprint peaks

whose area is larger than 90% of the total peak area.The similarity evaluation of 13 batches samples of P.cuspidate was more than 0.9.Conclution: The HPLC chromatographic fingerprint of P.cuspidate can express the chemical information completely and provide a scientific basis for controlling the quality of P.cuspidate.