硒化壳聚糖对慢性粒细胞白血病K562细胞bcr/abl融合基因表达的影响

邓守恒; 李芳; 李林均; 王贤和; 陈萍; 徐华; 潘东风

doi:10.13422/j.cnki.syfjx.2011.01.043

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硒化壳聚糖对慢性粒细胞白血病K562细胞bcr/abl融合基因表达的影响

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- 硒化壳聚糖对慢性粒细胞白血病K562细胞bcr/abl融合基因表达的影响
- Effect of Selenium Chitosan on Expression of bcr /abl Fusion Gene in Human Chronic Myelogenous Leukemia Cell Line K562
- 中国实验方剂学杂志 2011年17卷第1期页码：106-109
- 作者机构：
  
  1. 郧阳医学院附属人民医院肿瘤中心
  2. 十堰市妇幼保健院
- 作者简介：
- 基金信息：
  
  湖北省卫生厅基金（QJX2008-6）;郧阳医学院基金（2008QDJ1）
- DOI：10.13422/j.cnki.syfjx.2011.01.043
  中图分类号： R285.5
- 纸质出版日期：2011
- 稿件说明：
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[1]邓守恒,李芳,李林均,王贤和,陈萍,徐华,潘东风.硒化壳聚糖对慢性粒细胞白血病K562细胞bcr/abl融合基因表达的影响[J].中国实验方剂学杂志,2011,17(01):106-109.

DENG Shou-heng1, LI Fang1, LI Lin-jun1, et al. Effect of Selenium Chitosan on Expression of bcr /abl Fusion Gene in Human Chronic Myelogenous Leukemia Cell Line K562[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 106-109.
[1]邓守恒,李芳,李林均,王贤和,陈萍,徐华,潘东风.硒化壳聚糖对慢性粒细胞白血病K562细胞bcr/abl融合基因表达的影响[J].中国实验方剂学杂志,2011,17(01):106-109. DOI： 10.13422/j.cnki.syfjx.2011.01.043.

DENG Shou-heng1, LI Fang1, LI Lin-jun1, et al. Effect of Selenium Chitosan on Expression of bcr /abl Fusion Gene in Human Chronic Myelogenous Leukemia Cell Line K562[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 106-109. DOI： 10.13422/j.cnki.syfjx.2011.01.043.

摘要

目的:研究硒化壳聚糖对慢性粒细胞白血病K562细胞增殖的影响

探讨这种作用与bcr/abl融合基因表达的关系。方法:应用磺酰罗丹明B（SRB）法和集落形成法检测硒化壳聚糖对细胞增殖的影响;应用Western blot（免疫印迹）法检测细胞P210bcr/abl融合蛋白含量;应用反转录聚合酶链式反应（RT-PCR）法检测bcr/abl融合基因信使RNA（mRNA）水平。结果:50

100

200 mg·L-1硒化壳聚糖作用K562细胞48 h可明显抑制细胞增殖和降低其存活率（P<0.05

P<0.01）;50

100

200 mg·L-1硒化壳聚糖作用48 h或200 mg·L-1硒化壳聚糖作用12

48 h后K562细胞内P210bcr/abl蛋白含量呈时间和剂量依赖性下降（P<0.05

P<0.01）

而bcr/abl融合基因mRNA水平未见明显改变。结论:硒化壳聚糖可通过下调bcr/abl融合基因表达的P210bcr/abl融合蛋白对K562细胞增殖产生抑制作用。

Abstract

Objective: To investigate the effects of selenium chitosan（Sc） on proliferation of human chronic myelogenous leukemia cell line K562 and explore its relationship with the expression of bcr /abl fusion gene.Method: After treatment with Sc

cell proliferation was determined with SRB and clonal assay.The abundance of P210bcr/ablprotein was examined with Western blotting.The expression of bcr /abl fusion gene was detected by RT-PCR.Result: Exposure of K562 cells to 50

100 and 200 mg·L-1 of Sc

respectively

for 48h could obviously inhibit proliferation and decrease their survival rate（P < 0.05

P < 0.01）;After treatment with 50

100

200 mg·L-1 of Sc

respectively

for 48 h or 200 mg.L-1 of Sc for 12

and 48 h

the abundance of P210bcr/ablprotein in K562 cells was down-regulated in a concentration and time-dependent manner（P < 0.05

P < 0.01）

but the expression of bcr /abl fusion gene was not changed.Conclusions: Sc could inhibit the proliferation of K562 cells cultured in vitro

and this effect was correlated with down-regulation of the abundance of P210bcr/abl proteins encoded by bcr /abl fusion gene.

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